CAJ | 학술논문

目的：探讨血管内皮生长因子（VEGF）和凋亡抑制基因Bag-1、Bcl-2在肝癌细胞、癌旁及正常肝细胞中的表达，以及其与肝细胞癌预后的相关性。方法利用免疫组织化学染色法分别检测60例癌组织、30例癌旁组织及14例正常肝组织中VEGF、Bag-1及Bcl-2的水平，结合临床病理特征及预后对所得数据进行统计学分析。结果 VEGF、Bag-1及Bcl-2在肝癌组织中的表达显著高于癌旁和正常肝组织（P均＜0.05）；VEGF、Bag-1及Bcl-2的表达与肝癌的分期、有无淋巴结转移、有无癌栓形成以及有无包膜形成相关，差异均具有统计学意义（P均＜0.05）；与患者性别、年龄、肿瘤分级及大小以及甲胎蛋白（AFP）等因素无关。VEGF、Bag-1及Bcl-2的表达呈高度一致性（P＜0.05）；VEGF、Bag-1及Bcl-2阳性患者的1年和2年生存率均显著低于阴性患者（P＜0.05）。结论 VEGF、Bag-1及Bcl-2与肝癌的生物学行为密切相关，并可作为肝细胞癌患者预后的预测因素。
목적：탐토혈관내피생장인자（VEGF）화조망억제기인Bag-1、Bcl-2재간암세포、암방급정상간세포중적표체，이급기여간세포암예후적상관성。방법이용면역조직화학염색법분별검측60례암조직、30례암방조직급14례정상간조직중VEGF、Bag-1급Bcl-2적수평，결합림상병리특정급예후대소득수거진행통계학분석。결과 VEGF、Bag-1급Bcl-2재간암조직중적표체현저고우암방화정상간조직（P균＜0.05）；VEGF、Bag-1급Bcl-2적표체여간암적분기、유무림파결전이、유무암전형성이급유무포막형성상관，차이균구유통계학의의（P균＜0.05）；여환자성별、년령、종류분급급대소이급갑태단백（AFP）등인소무관。VEGF、Bag-1급Bcl-2적표체정고도일치성（P＜0.05）；VEGF、Bag-1급Bcl-2양성환자적1년화2년생존솔균현저저우음성환자（P＜0.05）。결론 VEGF、Bag-1급Bcl-2여간암적생물학행위밀절상관，병가작위간세포암환자예후적예측인소。
Objective To study the expression of vascular endothelial growth factor (VEGF), Bag-1 and Bcl-2 in hepatocellular carcinoma, para-tumor and normal liver tissue and to investigate the correlation of the presence of VEGF, Bag-1 and Bcl-2 with the prognosis of hepatocellular carcinoma. Methods The expressions of VEGF, Bag-1 and Bcl-2 proteins were detected by immunohistochemical stain in primary liver cancer (PLC) (60 cases), para-cancer (30 cases) and normal liver tissue samples (14 cases). Combined with the clinical pathological characteristics and prognosis of the data were statistically analyzed. Results Immunohistochemical analysis revealed that expression rates of VEGF, Bag-1 and Bcl-2 in PLC tissues were signiifcantly higher than that in para-cancer tissues and normal liver tissues (all P<0.05). The presence of VEGF, Bag-1 and Bcl-2 in the cancer cells were associated with clinical stage, with or without metastasis in lymph node, with or without capsual in tumor, with or without tumor embolus, all with signiifcant differences (all P<0.05). However, there was no signiifcant difference with gender, age, tumor differentiation and size, and AFP (all P > 0.05). The expression of VEGF, Bag-1 and Bcl-2 had high degree of consistency. The 1-year and 2-year survival rates in patients with VEGF, Bag-1 and Bcl-2 positive were signiifcantly decreased compared with patients of VEGF, Bag-1 and Bcl-2 negative (P<0.05). Conclusions There was a closely relationship between VEGF, Bag-1, Bcl-2 and clinicopathological characteristics of PLC, and it may serve a prognostic factor for PLC.