中华实验和临床感染病杂志（电子版）
中華實驗和臨床感染病雜誌（電子版）
중화실험화림상감염병잡지（전자판）
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2014年
2期
173-177
,共5页
程兆晶%周宝桐%张丽帆%侍效春%韩扬%张月秋%徐虹%刘晓清
程兆晶%週寶桐%張麗帆%侍效春%韓颺%張月鞦%徐虹%劉曉清
정조정%주보동%장려범%시효춘%한양%장월추%서홍%류효청
肝炎,乙型,慢性%树突状细胞%干扰素α%免疫
肝炎,乙型,慢性%樹突狀細胞%榦擾素α%免疫
간염,을형,만성%수돌상세포%간우소α%면역
Chronic hepatitis B%Dentritic cell%Interferon-alpha (IFN-α)%Immunity
目的：旨在检测慢性HBV感染者外周血单个核细胞（PBMC）分泌干扰素α（IFN-α）的情况，以探究其外周血浆细胞样树突状细胞（pDC）和单核细胞功能是否存在缺陷。方法前瞻性纳入2012年7月至2013年3月北京协和医院肝炎门诊就诊的慢性乙型肝炎（CHB）患者16例，同期选取与之年龄相匹配的HBV携带者16例和健康人18例。采集血样，分离PBMC，分别与含未甲基化的胞嘧啶鸟嘌呤二核苷酸序列的寡脱氧核苷酸2216（CPG ODN2216）、聚肌苷酸胞苷酸（poly I：C）刺激共培养，并用酶联免疫吸附试验（ELISA）测量其分泌IFN-α量。结果经ODN2216刺激后，CHB组患者PBMC分泌IFN-α量平均为31.20（7.33～44.04）pg/ml，HBV携带者组PBMC分泌IFN-α量平均为109.91（13.74～240.27）pg/ml，健康对照组PBMC分泌IFN-α量平均为107.95（48.59～227.33）。CHB患者组显著低于健康对照组，差异具有统计学意义（Z=-2.691，P=0.007）。CHB患者组显著低于HBV携带者组，差异具有统计学意义（Z=-2.206，P=0.027）。经poly（I：C）刺激后，三组PBMC分泌IFN-α量差异无统计学意义（F=0.628，P=0.427）。结论较HBV携带者和健康对照组，CHB患者体内PBMC中树突状细胞分泌IFN-α明显下降，而单核细胞分泌IFN-α量无显著性降低。
目的：旨在檢測慢性HBV感染者外週血單箇覈細胞（PBMC）分泌榦擾素α（IFN-α）的情況，以探究其外週血漿細胞樣樹突狀細胞（pDC）和單覈細胞功能是否存在缺陷。方法前瞻性納入2012年7月至2013年3月北京協和醫院肝炎門診就診的慢性乙型肝炎（CHB）患者16例，同期選取與之年齡相匹配的HBV攜帶者16例和健康人18例。採集血樣，分離PBMC，分彆與含未甲基化的胞嘧啶鳥嘌呤二覈苷痠序列的寡脫氧覈苷痠2216（CPG ODN2216）、聚肌苷痠胞苷痠（poly I：C）刺激共培養，併用酶聯免疫吸附試驗（ELISA）測量其分泌IFN-α量。結果經ODN2216刺激後，CHB組患者PBMC分泌IFN-α量平均為31.20（7.33～44.04）pg/ml，HBV攜帶者組PBMC分泌IFN-α量平均為109.91（13.74～240.27）pg/ml，健康對照組PBMC分泌IFN-α量平均為107.95（48.59～227.33）。CHB患者組顯著低于健康對照組，差異具有統計學意義（Z=-2.691，P=0.007）。CHB患者組顯著低于HBV攜帶者組，差異具有統計學意義（Z=-2.206，P=0.027）。經poly（I：C）刺激後，三組PBMC分泌IFN-α量差異無統計學意義（F=0.628，P=0.427）。結論較HBV攜帶者和健康對照組，CHB患者體內PBMC中樹突狀細胞分泌IFN-α明顯下降，而單覈細胞分泌IFN-α量無顯著性降低。
목적：지재검측만성HBV감염자외주혈단개핵세포（PBMC）분비간우소α（IFN-α）적정황，이탐구기외주혈장세포양수돌상세포（pDC）화단핵세포공능시부존재결함。방법전첨성납입2012년7월지2013년3월북경협화의원간염문진취진적만성을형간염（CHB）환자16례，동기선취여지년령상필배적HBV휴대자16례화건강인18례。채집혈양，분리PBMC，분별여함미갑기화적포밀정조표령이핵감산서렬적과탈양핵감산2216（CPG ODN2216）、취기감산포감산（poly I：C）자격공배양，병용매련면역흡부시험（ELISA）측량기분비IFN-α량。결과경ODN2216자격후，CHB조환자PBMC분비IFN-α량평균위31.20（7.33～44.04）pg/ml，HBV휴대자조PBMC분비IFN-α량평균위109.91（13.74～240.27）pg/ml，건강대조조PBMC분비IFN-α량평균위107.95（48.59～227.33）。CHB환자조현저저우건강대조조，차이구유통계학의의（Z=-2.691，P=0.007）。CHB환자조현저저우HBV휴대자조，차이구유통계학의의（Z=-2.206，P=0.027）。경poly（I：C）자격후，삼조PBMC분비IFN-α량차이무통계학의의（F=0.628，P=0.427）。결론교HBV휴대자화건강대조조，CHB환자체내PBMC중수돌상세포분비IFN-α명현하강，이단핵세포분비IFN-α량무현저성강저。
Objective To investigate the features of peripheral blood mononuclear cell (PBMC) secreting interferon-alfa (IFN-α) and evaluate the function of pDC and monocyte to produce IFN-αin patients with chronic hepatitis B virus infection. Methods The fresh blood samples were collected from 16 patients with chronic hepatitis B, 16 age matched patients with HBV carrier and 18 age matched healthy individuals as controls from July in 2012 to March in 2013 in Peking Union Medical College Hospital. PBMCs were isolated, respectively and then stimulated in vitro by CPG oligodeoxynucleotides (CPG ODN)2216 or polyinosinic-polycytidylic acid (poly I:C) as stimulators and measured by enzyme linked immunosorbent assay (ELISA) for IFN-α production. Results With CPG ODN2216 stimulation, the level of IFN-αproduction was 31.20 (7.33-44.04) pg/ml in CHB group, 109.91 (13.74-240.27) pg/ml in HBV carrier group and 107.95 (48.59-227.33) pg/ml in healthy control group, respectively. Lower levels of IFN-αproduction in CHB group were observed comparing with in healthy control group (Z=-2.691,P=0.007), and with in HBV carrier group (Z=-2.206,P=0.027). There were no signiifcant differences between the three groups (F=0.628,P=0.427) with poly (I:C) stimulation. Conclusions There was no signiifcant difference in IFN-αproduction from PBMCs pulsed with CPG ODN 2216 between HBV carrier group and healthy control group. Impaired function of pDC secreting IFN-αwas found in the patients with CHB, while no signiifcant difference in monocyte secreting. It suggested immune suppression is present in patients with CHB.
