CAJ | 학술논문

目的：比较脂多糖（LPS）气管滴注法与雾化吸入法建立的小鼠急性肺损伤（ALI）模型，确立更为有效的小鼠肺损伤建模方法。方法20只健康雄性C57BL/6小鼠随机分为生理盐水（NS）气管滴注组、NS雾化吸入组、LPS气管滴注组和LPS雾化吸入组。分别采用气管滴注法和雾化吸入法建立肺损伤模型，5 h后进行小鼠肺湿重/干重（W/D）比值测定、支气管肺泡灌洗液（BALF）蛋白含量测定、细胞分类计数及炎性细胞因子检测。结果与对照组相比，LPS气管滴注组和LPS雾化吸入组小鼠肺W/D比值，BALF中总蛋白浓度，TNF-α、IL-6、MCP-1和IL-1β等多项炎性细胞因子以及中性粒细胞数目均显著升高（P均＜0.05）；NS气管滴注组较NS雾化吸入组炎症背景高；LPS气管滴注组较LPS雾化吸入组各项肺部炎症指标组内差异大。结论雾化吸入方法对于建立小鼠ALI模型更有效。
목적：비교지다당（LPS）기관적주법여무화흡입법건립적소서급성폐손상（ALI）모형，학립경위유효적소서폐손상건모방법。방법20지건강웅성C57BL/6소서수궤분위생리염수（NS）기관적주조、NS무화흡입조、LPS기관적주조화LPS무화흡입조。분별채용기관적주법화무화흡입법건립폐손상모형，5 h후진행소서폐습중/간중（W/D）비치측정、지기관폐포관세액（BALF）단백함량측정、세포분류계수급염성세포인자검측。결과여대조조상비，LPS기관적주조화LPS무화흡입조소서폐W/D비치，BALF중총단백농도，TNF-α、IL-6、MCP-1화IL-1β등다항염성세포인자이급중성립세포수목균현저승고（P균＜0.05）；NS기관적주조교NS무화흡입조염증배경고；LPS기관적주조교LPS무화흡입조각항폐부염증지표조내차이대。결론무화흡입방법대우건립소서ALI모형경유효。
Objective To compare the mouse models of acute lung injury (ALI) established by intratracheal instillation (IT) and aerosol inhalation (IH) of lipopolysaccharide (LPS), and to investigate which method was more effective. Methods Twenty male mice were randomly divided into normal saline (NS)-IT group, NS-IH group, LPS-IT group and LPS-IH group. The mice of LPS-IT and LPS-IH groups were exposed to LPS via intratracheal instillation and aerosol inhalation, respectively. The analysis of lung wet/dry weight (W/D) ratio, total protein concentration of bronchoalveolar lavage fluid (BALF), inflammatory cytokines in BALF and multiple cell countings in BALF were performed 5 h after exposed. Results The lung W/D ratio, total protein concentration of BALF, inlfammatory cytokines such as TNF-α, IL-6, MCP-1 and IL-1βin BALF, and the amount of neutrophils in BALF were signiifcantly increased in the LPS-IT and LPS-IH groups compared with the NS-IT and NS-IH groups (P all < 0.05). Compared with the intratracheal instillation method, the aerosol inhalation method appeared to have lower background inlfammation in the controls and less inter-animal variability. Conclusion The aerosol inhalation is proved to be more effective to establish the mouse model of ALI.