CAJ | 학술논문

离体条件下，通过不同致病力溃疡病病菌、接种浓度、培养湿度、接种方式条件下接种红阳猕猴桃枝条，探讨不同条件下溃疡病病原菌对猕猴桃枝条的侵染效果。在此基础上，通过对24个猕猴桃品种（系）枝条人工接种溃疡病病原菌，评价不同猕猴桃品种对溃疡病的抗性。结果表明：强致病力病菌、高浓度、高湿度、枝条创伤面积都能显著影响猕猴桃枝条的病情指数；不同猕猴桃品种对溃疡病的抗性存在显著差异，24个猕猴桃品种（系）枝条中，华特和徐香表现为高抗；迷你华特、金魁、绿肉优系（ G-HZ201201）和毛雄表现为抗病；红阳、黄肉优系11-7、大红、早鲜、早艳和黄肉优系Y-HZ201201表现为高感。通过对华特、布鲁诺和红阳3个品种猕猴桃盆栽苗接种溃疡病病原菌，并测定接种植株与对照植株的相关生理参数，评价不同猕猴桃品种对溃疡病的抗性及其相关生理参数与抗溃疡病的关系。结果表明，3个品种盆栽苗的抗性评价等级与离体枝条接种基本一致。不同抗性猕猴桃品种酶活性与其病情指数呈线性关系，健康植株韧皮部中CAT酶活性与病情指数的相关性最高（ r＝0.999*），叶片中的CAT酶活性次之（ r＝0.994*），健康植株韧皮部和叶片中POD酶活性与病情指数的相关性分别为r＝0.979和r＝0.969，SOD酶活性分别为r＝0.924和r＝0.938。可溶性糖、可溶性淀粉、可溶性蛋白含量与病情指数也表现为线性关系，可溶性蛋白含量与病情指数相关性最高，在健康植株韧皮部和叶片的相关性分别为r＝-0.972和r＝-0.962，可溶性糖含量与病情指数的相关性分别为r＝0.934和r＝0.940。综上所述，不同猕猴桃品种对溃疡病的抗性存在显著性差异， CAT、POD、SOD酶活性和可溶性蛋白、可溶性糖含量可以作为鉴定猕猴桃抗溃疡病的生理指标。離體條件下，通過不同緻病力潰瘍病病菌、接種濃度、培養濕度、接種方式條件下接種紅暘獼猴桃枝條，探討不同條件下潰瘍病病原菌對獼猴桃枝條的侵染效果。在此基礎上，通過對24箇獼猴桃品種（繫）枝條人工接種潰瘍病病原菌，評價不同獼猴桃品種對潰瘍病的抗性。結果錶明：彊緻病力病菌、高濃度、高濕度、枝條創傷麵積都能顯著影響獼猴桃枝條的病情指數；不同獼猴桃品種對潰瘍病的抗性存在顯著差異，24箇獼猴桃品種（繫）枝條中，華特和徐香錶現為高抗；迷妳華特、金魁、綠肉優繫（ G-HZ201201）和毛雄錶現為抗病；紅暘、黃肉優繫11-7、大紅、早鮮、早豔和黃肉優繫Y-HZ201201錶現為高感。通過對華特、佈魯諾和紅暘3箇品種獼猴桃盆栽苗接種潰瘍病病原菌，併測定接種植株與對照植株的相關生理參數，評價不同獼猴桃品種對潰瘍病的抗性及其相關生理參數與抗潰瘍病的關繫。結果錶明，3箇品種盆栽苗的抗性評價等級與離體枝條接種基本一緻。不同抗性獼猴桃品種酶活性與其病情指數呈線性關繫，健康植株韌皮部中CAT酶活性與病情指數的相關性最高（ r＝0.999*），葉片中的CAT酶活性次之（ r＝0.994*），健康植株韌皮部和葉片中POD酶活性與病情指數的相關性分彆為r＝0.979和r＝0.969，SOD酶活性分彆為r＝0.924和r＝0.938。可溶性糖、可溶性澱粉、可溶性蛋白含量與病情指數也錶現為線性關繫，可溶性蛋白含量與病情指數相關性最高，在健康植株韌皮部和葉片的相關性分彆為r＝-0.972和r＝-0.962，可溶性糖含量與病情指數的相關性分彆為r＝0.934和r＝0.940。綜上所述，不同獼猴桃品種對潰瘍病的抗性存在顯著性差異， CAT、POD、SOD酶活性和可溶性蛋白、可溶性糖含量可以作為鑒定獼猴桃抗潰瘍病的生理指標。
리체조건하，통과불동치병력궤양병병균、접충농도、배양습도、접충방식조건하접충홍양미후도지조，탐토불동조건하궤양병병원균대미후도지조적침염효과。재차기출상，통과대24개미후도품충（계）지조인공접충궤양병병원균，평개불동미후도품충대궤양병적항성。결과표명：강치병력병균、고농도、고습도、지조창상면적도능현저영향미후도지조적병정지수；불동미후도품충대궤양병적항성존재현저차이，24개미후도품충（계）지조중，화특화서향표현위고항；미니화특、금괴、록육우계（ G-HZ201201）화모웅표현위항병；홍양、황육우계11-7、대홍、조선、조염화황육우계Y-HZ201201표현위고감。통과대화특、포로낙화홍양3개품충미후도분재묘접충궤양병병원균，병측정접충식주여대조식주적상관생리삼수，평개불동미후도품충대궤양병적항성급기상관생리삼수여항궤양병적관계。결과표명，3개품충분재묘적항성평개등급여리체지조접충기본일치。불동항성미후도품충매활성여기병정지수정선성관계，건강식주인피부중CAT매활성여병정지수적상관성최고（ r＝0.999*），협편중적CAT매활성차지（ r＝0.994*），건강식주인피부화협편중POD매활성여병정지수적상관성분별위r＝0.979화r＝0.969，SOD매활성분별위r＝0.924화r＝0.938。가용성당、가용성정분、가용성단백함량여병정지수야표현위선성관계，가용성단백함량여병정지수상관성최고，재건강식주인피부화협편적상관성분별위r＝-0.972화r＝-0.962，가용성당함량여병정지수적상관성분별위r＝0.934화r＝0.940。종상소술，불동미후도품충대궤양병적항성존재현저성차이， CAT、POD、SOD매활성화가용성단백、가용성당함량가이작위감정미후도항궤양병적생리지표。
In this study , the infection effect of canker pathogens was determined by the inoculation of the branches of Hongyang kiwifruit in vitro under different pathogenicity , inoculation concentration , cultivation humidity , and inoculation methods .Then, the resistance of 24 kiwifruit varieties were evaluated .The result showed as follows:pathogens with high pathogenicity ,high concentration,high humidity,and wound area of branch all had remarkable effect on the disease index of kiwifruit branches;Different kiwifruit varieties showed different resistance to canker . Among the branches of 24 varieties,White and Xuxiang showed high resistance;Miniwhite,Jinqui,Excellentgreenflesh (G-HZ201201) and Maoxiong showed resistance;Dahong,Zaoxian,Zaoyan and Excellentyellowflesh ( Y-HZ201201 ) showed highly susceptible characteristic .In addition, the potted seedlings of White , Bruno and Hongyang were inoculated with canker pathogens and the related physiological parameters between inoculated plants and control plants were measured .The result showed that the resistance rank of 3 varieties with potted seedlings was correspond to that with blanches in vitro.Enzymatic activity of kiwifruit varieties with different resistance had a linear relationship with their disease index .CAT enzyme activity in phloem of healthy plants had the highest relevancy ( r=0.999*) , and CAT enzyme activity of blades took the second place ( r=0.994*) .Relevancies between disease index and POD enzyme activity of healthy plants phloem and blades were r =0.979 and r=0.969 respectively .The relevancy of SOD enzyme activity were r=-0.924 and r=-0.938 .Soluble sugar ,soluble starch and soluble protein also had a linear relation with disease index .Soluble protein had the highest relevancy with disease index of r =-0.972 and r =-0.962 in healthy plants phloem and blades .The relevancy between soluble sugar and disease index was r =-0.934 and r=-0.940.To sum up,the resistance of different varieties of kiwifruit to canker had a significant difference,and the activity of CAT,POD and SOD, as well as the content of soluble protein and soluble sugar could be used as the physiological index in the identification of kiwifruit resistance to canker .