CAJ | 학술논문

利用亚细胞定位技术对水稻抗白叶枯病相关基因的表达产物进行了分析，为基因功能分析提供了重要信息。通过前期基因芯片筛选、半定量/定量PCR的验证及基因功能注释，从高抗白叶枯病水稻品种Y73中筛选了18个潜在的与抗白叶枯病相关的基因，并在烟草叶片细胞中进行亚细胞定位分析。通过观察融合有绿色荧光标记（ sGFP）的表达产物，初步了解这些基因在烟草细胞中的表达位置。经过激光共聚焦显微镜观察后发现：其中4个基因的 sGFP 融合蛋白定位于细胞核上，可能发挥了转录因子的功能；4个基因的 sGFP 融合蛋白定位于细胞质膜上，推测可能与细胞质膜的稳定或控制蛋白转运相关；4个基因的 sGFP 融合蛋白同时定位于细胞核和细胞质膜上；另有6个基因的 sGFP 融合表达后没有观察到明显的定位信号。
이용아세포정위기술대수도항백협고병상관기인적표체산물진행료분석，위기인공능분석제공료중요신식。통과전기기인심편사선、반정량/정량PCR적험증급기인공능주석，종고항백협고병수도품충Y73중사선료18개잠재적여항백협고병상관적기인，병재연초협편세포중진행아세포정위분석。통과관찰융합유록색형광표기（ sGFP）적표체산물，초보료해저사기인재연초세포중적표체위치。경과격광공취초현미경관찰후발현：기중4개기인적 sGFP 융합단백정위우세포핵상，가능발휘료전록인자적공능；4개기인적 sGFP 융합단백정위우세포질막상，추측가능여세포질막적은정혹공제단백전운상관；4개기인적 sGFP 융합단백동시정위우세포핵화세포질막상；령유6개기인적 sGFP 융합표체후몰유관찰도명현적정위신호。
The subcellular localization of rice bacterial blight related genes were examined to provide important information for the study of gene function .Eighteen candidate genes which potentially associated with bacterial blight resistance were selected from Y73 by using gene chip, semi-quantitative/quantitative PCR and function annotation . These candidate genes were fused with green fluorescent marker ( sGFP) and subcellular localization of their products in tobacco leaf cells .Four genes combined with sGFP were found mainly located in the cell nucleus , showing the typical characteristics of transcription factors; Four genes were found mainly located in the plasma membrane , indicating their potential functions of maintaining the stability of the cell membrane or in transport control on cell membrane;Four genes were simultaneously located in both cell nucleus and the plasma membrane and there was no apparent subcellular localization signal for the remaining 6 genes combined with sGFP .