CAJ | 학술논문

观察淫羊藿和富血小板血浆（ PRP）对SD大鼠软骨细胞增殖作用的影响，探讨两者对软骨细胞的协同作用。取3周龄SD大鼠的膝关节软骨建立软骨细胞体外培养体系，分别采用MTT法、流式细胞术对细胞增殖作用进行检测。结果表明：（1） PRP对软骨细胞增殖有显著的促进作用（P＜0.01）；（2）淫羊藿对软骨细胞有一定的增殖作用，但不显著；（3）淫羊藿联合PRP对软骨细胞的增殖作用显著下降，效果低于淫羊藿或PRP组（ P＜0.01）。淫羊藿联合PRP对软骨细胞有显著抑制作用（ P＜0.01），推断淫羊藿某些成分抑制了PRP中生长因子的活性，从而减弱了PRP对软骨细胞的作用，该推断有待进一步研究确认。
관찰음양곽화부혈소판혈장（ PRP）대SD대서연골세포증식작용적영향，탐토량자대연골세포적협동작용。취3주령SD대서적슬관절연골건립연골세포체외배양체계，분별채용MTT법、류식세포술대세포증식작용진행검측。결과표명：（1） PRP대연골세포증식유현저적촉진작용（P＜0.01）；（2）음양곽대연골세포유일정적증식작용，단불현저；（3）음양곽연합PRP대연골세포적증식작용현저하강，효과저우음양곽혹PRP조（ P＜0.01）。음양곽연합PRP대연골세포유현저억제작용（ P＜0.01），추단음양곽모사성분억제료PRP중생장인자적활성，종이감약료PRP대연골세포적작용，해추단유대진일보연구학인。
To observe the proliferative effects of Epimedium brevicornum Maxim and platelet-rich plasma ( PRP), respectively, on SD rat chondrocyte , and to explore their synergic effect between each other , in vitro chondrocyte culter system was established using 3-week-old SD rat knee cartilage .MTT and flow cytometry assays were adopted to test the chondrocyte prolifereation .Results:(1) PRP has significant proliferative effect on chondrocyte (P<0.01);(2) Epimedium brevicornum Maxim has a bit proliferative effect on chondrocyte;(3) Epimedium brevicornum Maxim combined with PRP has inhibitory effect on chondrocyte ( P<0.01 ) .Epimedium brevicornum Maxim combined PRP has inhibitory effect on chondrocyte (P<0.01), deducing that some components in Epimedium brevicornum Maxim inhibited PRP growth factors and then decrease PRP activity .The conclusion needs further investigation .