CAJ | 학술논문

为了进一步完善大丽花脱毒技术，获得优质大丽花脱毒苗，以感染大丽花花叶病毒（ DMV）、黄瓜花叶病毒（CMV-D2）和烟草花叶病毒（TMV）的大丽花为试材，采用茎尖结合热处理、化学处理方法对大丽花脱毒效果进行了探讨。各培养单株经酶联免疫法（ ELISA ）检测，结果表明，茎尖结合热处理能有效脱除DMV和CMV-D2，脱毒率分别达到74.7％和66.9％，但不能有效脱除TMV，对3种病毒均脱除的比率为10.6％；茎尖结合化学处理能有效脱除DMV，CMV-D2和TMV，脱除率分别为83.1％，80.5％和77.1％，对3种病毒的脱除率为24.3％。
위료진일보완선대려화탈독기술，획득우질대려화탈독묘，이감염대려화화협병독（ DMV）、황과화협병독（CMV-D2）화연초화협병독（TMV）적대려화위시재，채용경첨결합열처리、화학처리방법대대려화탈독효과진행료탐토。각배양단주경매련면역법（ ELISA ）검측，결과표명，경첨결합열처리능유효탈제DMV화CMV-D2，탈독솔분별체도74.7％화66.9％，단불능유효탈제TMV，대3충병독균탈제적비솔위10.6％；경첨결합화학처리능유효탈제DMV，CMV-D2화TMV，탈제솔분별위83.1％，80.5％화77.1％，대3충병독적탈제솔위24.3％。
In order to improve the detoxification technology of Dahlia pinnata, acquire high-quality virus-free seedlings, Dahlia pinnata infected with dahlia mosaic virus (DMV), cucumber mosaic virus (CMV-D2) and tobacco mosaic virus ( TMV) were used as experimental material , the effects of heat treatment , chemical treatment methods combined with stem tip culture on detoxification were studied in this paper .The results of enzyme-linked immunoassay ( ELISA) showed that stem tip in combination with heat treatment could effectively remove the DMV and CMV-D2, and virus-free rate was 74.7% and 66.9% respectively, but it could not remove TMV effectively , while the removal rate of three kinds of viruses was 10.6%.Stem tip method combined with chemical treatment could effectively remove the DMV, CMV-D2 and TMV, and virus-free rate was 83.1%, 80.5%and 77.1%respectively, while virus-free rate of all the three was 24.3%.