CAJ | 학술논문

开发新型毛细管电泳-光纤光谱联用技术并应用于结晶紫的快速检测。以结晶紫为对象，考察了电泳和溶液条件对结晶紫的分离分析信号的影响。结果表明，检测结晶紫的最佳条件为：流动相是0．1 mol/L磷酸缓冲溶液（pH 4．0），检测波长590 nm，分离电压3 kV，进样时间60 s.在最佳条件下，吸光度与0．8～10μg/mL结晶紫呈现良好的线性关系，相关系数为0．9954，检出限为0．8μg/mL.方法成功地应用于水体中结晶紫的快速检测。
개발신형모세관전영-광섬광보련용기술병응용우결정자적쾌속검측。이결정자위대상，고찰료전영화용액조건대결정자적분리분석신호적영향。결과표명，검측결정자적최가조건위：류동상시0．1 mol/L린산완충용액（pH 4．0），검측파장590 nm，분리전압3 kV，진양시간60 s.재최가조건하，흡광도여0．8～10μg/mL결정자정현량호적선성관계，상관계수위0．9954，검출한위0．8μg/mL.방법성공지응용우수체중결정자적쾌속검측。
A novel method for the determination of crystal violet ( CV) has been developed based the capillary electrophoresis-fiber optic spectrometer system .The conditions for the system and analysis of CV are experimentally investigated .The opti-mum conditions are as follow:mobile phase, 0.1 mol/L H3PO4 -NaH2PO4 buffer solution (pH 4.0), detection wavelength at 590nm in the visible region, separation voltage 3000V, and injection time 60s.Under the optimum conditions, the absor-bance is proportional linearly to CV concentration in the range of 3 and 10 μg/mL, the correlation coefficient is 0.9954, and the detection limit was 0.8 μg/mL.This method has been successfully applied in rapid detection of CV .