中国稻米
中國稻米
중국도미
CHINA RICE
2014年
3期
30-35
,共6页
陈玲%殷富有%李维蛟%李定琴%张敦宇%钟巧芳%陈越%柯学%黄兴奇%程在全
陳玲%慇富有%李維蛟%李定琴%張敦宇%鐘巧芳%陳越%柯學%黃興奇%程在全
진령%은부유%리유교%리정금%장돈우%종교방%진월%가학%황흥기%정재전
野生稻%ISSR标记%亲缘关系%遗传多样性%遗传进化
野生稻%ISSR標記%親緣關繫%遺傳多樣性%遺傳進化
야생도%ISSR표기%친연관계%유전다양성%유전진화
wild rice%ISSR markers%phylogenetic relationship%genetic diversity%phylogeny evolution
采用ISSR标记，对云南三种野生稻遗传多样性进行研究，并以竹类、栽培稻粳稻02428和籼稻金刚30以及非洲长雄野生稻为对照进行聚类分析，探讨云南三种野生稻遗传进化方向。结果表明，33条ISSR引物，在所有的材料中总共扩增出464个等位基因，平均每条引物扩增出多态性条带14.1条；从总体水平上看，云南三种野生稻具有丰富的遗传多样性，三种野生稻的多态位点数NP、多态位点百分率p、有效等位基因数Na、观察等位基因数Ne、Nei基因多样性指数H和香农指数I分别为357、76.74%、1.7694±0.4217、1.3391±0.3637、0.2037±0.1851和0.3180±0.2532；三种野生稻品种间基因分化系数Gst为0.5194，品种间的基因流Nm为0.4627，说明三种野生稻之间基因交流水平低，品种间存在较大的遗传分化。 UPGMA聚类分析表明，在三种野生稻的进化过程中，疣粒野生稻分化最早，其次为药用野生稻，普通野生稻分化最晚。
採用ISSR標記，對雲南三種野生稻遺傳多樣性進行研究，併以竹類、栽培稻粳稻02428和秈稻金剛30以及非洲長雄野生稻為對照進行聚類分析，探討雲南三種野生稻遺傳進化方嚮。結果錶明，33條ISSR引物，在所有的材料中總共擴增齣464箇等位基因，平均每條引物擴增齣多態性條帶14.1條；從總體水平上看，雲南三種野生稻具有豐富的遺傳多樣性，三種野生稻的多態位點數NP、多態位點百分率p、有效等位基因數Na、觀察等位基因數Ne、Nei基因多樣性指數H和香農指數I分彆為357、76.74%、1.7694±0.4217、1.3391±0.3637、0.2037±0.1851和0.3180±0.2532；三種野生稻品種間基因分化繫數Gst為0.5194，品種間的基因流Nm為0.4627，說明三種野生稻之間基因交流水平低，品種間存在較大的遺傳分化。 UPGMA聚類分析錶明，在三種野生稻的進化過程中，疣粒野生稻分化最早，其次為藥用野生稻，普通野生稻分化最晚。
채용ISSR표기，대운남삼충야생도유전다양성진행연구，병이죽류、재배도갱도02428화선도금강30이급비주장웅야생도위대조진행취류분석，탐토운남삼충야생도유전진화방향。결과표명，33조ISSR인물，재소유적재료중총공확증출464개등위기인，평균매조인물확증출다태성조대14.1조；종총체수평상간，운남삼충야생도구유봉부적유전다양성，삼충야생도적다태위점수NP、다태위점백분솔p、유효등위기인수Na、관찰등위기인수Ne、Nei기인다양성지수H화향농지수I분별위357、76.74%、1.7694±0.4217、1.3391±0.3637、0.2037±0.1851화0.3180±0.2532；삼충야생도품충간기인분화계수Gst위0.5194，품충간적기인류Nm위0.4627，설명삼충야생도지간기인교류수평저，품충간존재교대적유전분화。 UPGMA취류분석표명，재삼충야생도적진화과정중，우립야생도분화최조，기차위약용야생도，보통야생도분화최만。
The genetic diversity in three wild rice species and bamboo, cultivated rice and O. longistaminata were assessed, and com-pared by ISSR method to determine their phylogenetic relationships, discuss the evolution direction of three kinds of Yunnan wild rice. The results indicated that a total of 464 alleles were detected among all the materials by 33 strips of ISSR primers, 14.1 polymorphic bands were amplified of the average of each primer. Rich genetic diversity existed in the three wild rice species at the aggregate level. The number of polymorphic loci(Np), the percentage of polymorphic loci(p), the effective number of alleles(Na), observed number of alleles(Ne), Nei 's genetic diversity index(H) and Shannon index(I) were 357, 76.74%, 1.7694±0.4217, 1.3391±0.3637, 0.2037± 0.1851 and 0.3180±0.2532. The coefficient of gene differentiation(Gst) was 0.0968 among the three wild rice species while the gene flow (Nm) was 0.4627, which shown that the gene exchanged at low level among three wild rice species and the genetic divergence was relatively high among rice varieties. UPGMA cluster analysis revealed the evolutionary process of three wild rice, O. meyeriana differentiats first, following by the O. Officinalis, O. rufipogon differentiats at the latest.
