植物营养与肥料学报
植物營養與肥料學報
식물영양여비료학보
PLANT NUTRITION AND FERTILIZER SCIENCE
2014年
3期
670-680
,共11页
张敏%梁国鹏%姜春辉%崔秀敏
張敏%樑國鵬%薑春輝%崔秀敏
장민%량국붕%강춘휘%최수민
番茄%铜胁迫%一氧化氮%Fe%Zn%Mn%亚细胞分布
番茄%銅脅迫%一氧化氮%Fe%Zn%Mn%亞細胞分佈
번가%동협박%일양화담%Fe%Zn%Mn%아세포분포
tomato seedlings%copper stress%nitric oxide iron zinc%manganese%subcellular distribution
采用营养液培养方法,以“改良毛粉802F1”番茄为材料,研究外源一氧化氮( NO,SNP为供体)对铜( Cu)胁迫下番茄幼苗铁( Fe)、锌( Zn)、锰( Mn)吸收分配的影响。结果显示,50μmol/L的Cu2+胁迫下,番茄幼苗的生物量和株高显著降低了33.7%和23.1%,外施100μmol/L SNP可显著缓解这种抑制作用,提高Cu胁迫下番茄幼苗根系、茎中Fe、Mn含量及叶柄、叶片中Fe、Zn含量,降低茎中Zn含量及叶柄、叶片中Mn含量;根系、茎、叶柄、叶片Fe、Zn及根系和茎中Mn的累积相应增加;根系吸收的Fe、Zn、Mn向地上部的转运降低。 Cu 胁迫下,外源NO可显著提高番茄液泡、细胞器的Fe、Zn含量,降低根系和叶片细胞壁Fe、Zn、Mn含量。在作为转运组织的茎和叶柄中,Mn主要分布在细胞壁上,而在叶柄和叶片液泡、细胞器中也有增加。表明外源NO可以调控番茄幼苗各部位及亚细胞中Fe、Zn、Mn的合理分布,维持胞质离子稳态和矿质营养元素平衡,缓解铜胁迫,保证番茄幼苗正常的生理代谢。
採用營養液培養方法,以“改良毛粉802F1”番茄為材料,研究外源一氧化氮( NO,SNP為供體)對銅( Cu)脅迫下番茄幼苗鐵( Fe)、鋅( Zn)、錳( Mn)吸收分配的影響。結果顯示,50μmol/L的Cu2+脅迫下,番茄幼苗的生物量和株高顯著降低瞭33.7%和23.1%,外施100μmol/L SNP可顯著緩解這種抑製作用,提高Cu脅迫下番茄幼苗根繫、莖中Fe、Mn含量及葉柄、葉片中Fe、Zn含量,降低莖中Zn含量及葉柄、葉片中Mn含量;根繫、莖、葉柄、葉片Fe、Zn及根繫和莖中Mn的纍積相應增加;根繫吸收的Fe、Zn、Mn嚮地上部的轉運降低。 Cu 脅迫下,外源NO可顯著提高番茄液泡、細胞器的Fe、Zn含量,降低根繫和葉片細胞壁Fe、Zn、Mn含量。在作為轉運組織的莖和葉柄中,Mn主要分佈在細胞壁上,而在葉柄和葉片液泡、細胞器中也有增加。錶明外源NO可以調控番茄幼苗各部位及亞細胞中Fe、Zn、Mn的閤理分佈,維持胞質離子穩態和礦質營養元素平衡,緩解銅脅迫,保證番茄幼苗正常的生理代謝。
채용영양액배양방법,이“개량모분802F1”번가위재료,연구외원일양화담( NO,SNP위공체)대동( Cu)협박하번가유묘철( Fe)、자( Zn)、맹( Mn)흡수분배적영향。결과현시,50μmol/L적Cu2+협박하,번가유묘적생물량화주고현저강저료33.7%화23.1%,외시100μmol/L SNP가현저완해저충억제작용,제고Cu협박하번가유묘근계、경중Fe、Mn함량급협병、협편중Fe、Zn함량,강저경중Zn함량급협병、협편중Mn함량;근계、경、협병、협편Fe、Zn급근계화경중Mn적루적상응증가;근계흡수적Fe、Zn、Mn향지상부적전운강저。 Cu 협박하,외원NO가현저제고번가액포、세포기적Fe、Zn함량,강저근계화협편세포벽Fe、Zn、Mn함량。재작위전운조직적경화협병중,Mn주요분포재세포벽상,이재협병화협편액포、세포기중야유증가。표명외원NO가이조공번가유묘각부위급아세포중Fe、Zn、Mn적합리분포,유지포질리자은태화광질영양원소평형,완해동협박,보증번가유묘정상적생리대사。
A tomato cultivar, Gailiang Maofen 802F1, was selected as the planting material to investigate effects of NO on the absorption and distribution of Fe , Zn and Mn in tomato seedlings under copper stress using nutrient solution cultivation in greenhouse .The results show that the biomass and plant height of tomato seedlings are decreased by 33.7% and 23.1% respectively under the 50 μmol/L copper stress , while the addition of 100μmol/L SNP could remarkably alleviate this inhibition effect . For mineral elements , SNP could remarkably increase the Fe and Mn concentrations in roots and stems , and Fe and Zn concentrations in petioles and leaves , and the Zn concentration in stems and the Mn concentrations in petioles and leaves are reduced under the copper stress . Meanwhile the exogenous NO could improve Fe and Zn accumulation amounts in roots , stems, petioles and leaves and Mn accumulation amounts in roots and stems , while the exogenous NO reduces the transformation of the absorbed Fe , Zn and Mn red from roots to shoots .Under the Cu stress , the exogenous NO could remarkably increase the Fe and Zn concentrations in vacuole and organelle of all organs and decrease Fe , Zn and Mn concentrations in cell wall of roots and leaves .Mn mainly concentrates on the cell walls of stems and petioles that function as transport organizations , while its concentrations in vacuole and organelle of petioles and leaves are also increased .These results suggest that the exogenous NO could effectively regulate Fe , Zn and Mn rational distributions in organs and subcellular microzone under the Cu stress , so that the seedlings could keep a better balance between mineral nutrition elements and intracellular ion homeostasis to maintain normal physiological metabolism of tomato.