CAJ | 학술논문

目的：探讨XS1000i血细胞分析仪部分血小板参数缺失时小红细胞/裂红细胞对血小板计数的影响。方法对XS1000i血细胞分析仪分析后MCV<80fl且部分血小板参数缺失的标本再使用PENTRA120Retic血细胞分析仪计数血小板、显微镜计数血小板及瑞氏染色镜检。结果 PLT>100×109/L时，XS1000i与PENTRA120Retic、显微镜计数血小板的差异有统计学意义(P<0.01)，PLT<100×109/L时, XS1000i与PENTRA120Retic、显微镜计数血小板的结果差异无统计学意义(P>0.01)；当MCV分别为70~80fl，60~70fl，<60fl时，XS1000i均高于显微镜计数血小板的结果,差异有统计学意义(P<0.01)。显微镜检查结果发现：101例标本均检出小红细胞或(和)裂红细胞，其中60例伴有大血小板或(和)巨血小板，4例伴有大血小板和血小板聚集。结论对于电阻抗血细胞分析仪，当MCV<80fl且部分血小板参数缺失时的标本应用显微镜或ICSH推荐的参考方法计数血小板，同时染色镜检异常血细胞的形态、数量及分布。
목적：탐토XS1000i혈세포분석의부분혈소판삼수결실시소홍세포/렬홍세포대혈소판계수적영향。방법대XS1000i혈세포분석의분석후MCV<80fl차부분혈소판삼수결실적표본재사용PENTRA120Retic혈세포분석의계수혈소판、현미경계수혈소판급서씨염색경검。결과 PLT>100×109/L시，XS1000i여PENTRA120Retic、현미경계수혈소판적차이유통계학의의(P<0.01)，PLT<100×109/L시, XS1000i여PENTRA120Retic、현미경계수혈소판적결과차이무통계학의의(P>0.01)；당MCV분별위70~80fl，60~70fl，<60fl시，XS1000i균고우현미경계수혈소판적결과,차이유통계학의의(P<0.01)。현미경검사결과발현：101례표본균검출소홍세포혹(화)렬홍세포，기중60례반유대혈소판혹(화)거혈소판，4례반유대혈소판화혈소판취집。결론대우전조항혈세포분석의，당MCV<80fl차부분혈소판삼수결실시적표본응용현미경혹ICSH추천적삼고방법계수혈소판，동시염색경검이상혈세포적형태、수량급분포。
Objective To investigate the effect of microcytes/schistocytes on platelet count when deficiency of portion platelet parameters measured by XS1000i hematology analyzer. Methods After analyzed by XS1000i hematology analyzer, the samples which have MCV<80fl and loss partial platelet parameters were re-measured with the PENTRA120Retic hematology analyzer, mi-croscopic platelet count and,Wright staining for platelets. Results When PLT>100×109/L, there were statistically significant differ-ences in the platelet counts among XS1000i, PENTRA120Retic and microscopic counting (P<0.01); when PLT<100×109/L, there were no statistically significant differences in the platelet counts among XS1000i PENTRA120Retic and microscopic counting (P>0.01). When MCV was 70~80fl, 60~70fl and<60fl, respectively, the platelet counts of XS1000i were higher than those of micro-scopic counting , the difference was statistically significant(P<0.01). The results of microscope examination were as follows:all 101 specimens were detected and had microcytes or (and) schistocytes, of which 60 cases were accompanied with large platelets and/or giant platelets, and 4 cases were accompanied with large platelets and platelet aggregation. Conclusions The samples with MCV<80fl and missing partial platelet parameters when analyzed by impedance hematology analyzer should be re-measured with the reference method recommended by ICSH or microscopic platelet counting, meanwhile, detect the morphology, number and distribu-tion of abnormal blood cells by staining and microscopic examination.