实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2014年
3期
243-245
,共3页
术后感染%小檗碱%产AmpC酶%抗菌作用
術後感染%小檗堿%產AmpC酶%抗菌作用
술후감염%소벽감%산AmpC매%항균작용
Postoperative infection%Berberine%AmpC enzyme%Antibacterial effect
目的：了解外科术后感染的病原菌分布情况并探讨小檗碱对产AmpC酶术后感染分离菌株的抗菌作用。方法从术后感染病人中分离出产AmpC酶细菌，并采用琼脂对倍稀释法、肉汤稀释法分别检测小檗碱对产AmpC酶三种分离菌株的最低抑菌作用。结果共分离出G-杆菌225株，其中产AmpC酶大肠埃希菌46株，有27株MIC为浓度31.25g/l小檗碱；高产AmpC酶肺炎克雷伯菌有26株，有13株MIC为浓度31.25g/l小檗碱；产AmpC铜绿假单胞菌21株，有12株MIC为浓度60.25g/l小檗碱。浓度为15.62g/L小檗碱对产AmpC酶分离菌基本上无抑制作用。G+的病原菌有170株，其中金黄色葡萄球菌94例，表皮葡萄球菌36例，真菌27例。结论产AmpC酶的G-杆菌在术后感染中较为常见，而小檗碱对产AmpC酶细菌株有一定的抑菌作用，值得临床上推广应用，以减少滥用抗生素而引起多重耐药的产生。
目的：瞭解外科術後感染的病原菌分佈情況併探討小檗堿對產AmpC酶術後感染分離菌株的抗菌作用。方法從術後感染病人中分離齣產AmpC酶細菌，併採用瓊脂對倍稀釋法、肉湯稀釋法分彆檢測小檗堿對產AmpC酶三種分離菌株的最低抑菌作用。結果共分離齣G-桿菌225株，其中產AmpC酶大腸埃希菌46株，有27株MIC為濃度31.25g/l小檗堿；高產AmpC酶肺炎剋雷伯菌有26株，有13株MIC為濃度31.25g/l小檗堿；產AmpC銅綠假單胞菌21株，有12株MIC為濃度60.25g/l小檗堿。濃度為15.62g/L小檗堿對產AmpC酶分離菌基本上無抑製作用。G+的病原菌有170株，其中金黃色葡萄毬菌94例，錶皮葡萄毬菌36例，真菌27例。結論產AmpC酶的G-桿菌在術後感染中較為常見，而小檗堿對產AmpC酶細菌株有一定的抑菌作用，值得臨床上推廣應用，以減少濫用抗生素而引起多重耐藥的產生。
목적：료해외과술후감염적병원균분포정황병탐토소벽감대산AmpC매술후감염분리균주적항균작용。방법종술후감염병인중분리출산AmpC매세균，병채용경지대배희석법、육탕희석법분별검측소벽감대산AmpC매삼충분리균주적최저억균작용。결과공분리출G-간균225주，기중산AmpC매대장애희균46주，유27주MIC위농도31.25g/l소벽감；고산AmpC매폐염극뢰백균유26주，유13주MIC위농도31.25g/l소벽감；산AmpC동록가단포균21주，유12주MIC위농도60.25g/l소벽감。농도위15.62g/L소벽감대산AmpC매분리균기본상무억제작용。G+적병원균유170주，기중금황색포도구균94례，표피포도구균36례，진균27례。결론산AmpC매적G-간균재술후감염중교위상견，이소벽감대산AmpC매세균주유일정적억균작용，치득림상상추엄응용，이감소람용항생소이인기다중내약적산생。
Objective To investigate the pathogenic bacteria distribution in postoperative infection and the effects of berberine on the antibacterial effect of AmpC enzyme producing strains. Methods The bacteria producing AmpC enzyme were isolated from patients with postoperative infection, and the minimum inhibitory effects of berberine on the three kinds of isolates producing AmpC enzymes were detected by using the agar dilution method and broth dilution method. Results A total of 225 strains of G-Bacillus were isolated ,46 strains of which producing AmpC enzyme, the MIC of 27 strains was 31.25g/L berberine;26 strains of Klebsiella pneumoniae producing high AmpC, 13 of which had MIC concentration of 31.25g/L berberine;21 strains of Pseudomonas aeruginosa producing AmpC, 12 strains of which had MIC concentration of 60.25g/L berberine. The concentration of 15.62g/L berberine had no inhibitory effect on bacteria producing AmpC enzyme. There were 170 strains of G+pathogen, including 94 cases of Staphylococcus aureus, 36 cases of Staphylococcus epidermidis and 27 cases of fungus. Conclusion G-coli producing AmpC enzyme in postoperative infections are more common, and the berberine has some antibacterial effect on bacterial strains producing AmpC enzyme. In order to reduce the abuse of antibiotics and cause multiple drug resistance, it is worthy of clinical application.
