CAJ | 학술논문

目的：研究D-氨基半乳糖联合脂多糖诱导小鼠慢性肝损伤模型建立的方法。方法30 mg/mLD-氨基半乳糖和2μg/mL脂多糖混合溶液，腹腔注射，每2天1次，连续8周。监测小鼠饮食和体重变化；取血测定小鼠血清丙氨酸转氨酶（ ALT）、天冬氨酸转氨酶（ AST）水平；取肝组织，HE和Masson染色，观察小鼠肝组织结构、细胞形态及纤维化程度。结果模型组ALT水平升高，肝细胞变性、坏死等病变，组织纤维化明显增生。结论D-氨基半乳糖联合脂多糖可诱导小鼠的慢性肝损伤模型。
목적：연구D-안기반유당연합지다당유도소서만성간손상모형건립적방법。방법30 mg/mLD-안기반유당화2μg/mL지다당혼합용액，복강주사，매2천1차，련속8주。감측소서음식화체중변화；취혈측정소서혈청병안산전안매（ ALT）、천동안산전안매（ AST）수평；취간조직，HE화Masson염색，관찰소서간조직결구、세포형태급섬유화정도。결과모형조ALT수평승고，간세포변성、배사등병변，조직섬유화명현증생。결론D-안기반유당연합지다당가유도소서적만성간손상모형。
Objective To research the method of Chronic hepatic injury modeling in mice induced by D -galactosamine and lipopolysaccharide combination . Methods Injected D-galactosamine ( 30 mg/mL ) and lipopolysaccharide ( 2μg/mL ) combination by intraperitoneal injection , two days at a time for 8 weeks .Monitored variation of diet and weight; detected serum level of alanine aminotransferase ( ALT ) and aspartate aminotransferase (AST), been put to death in mice and removed the liver tissue .strained hepatic tissue by the HE and Masoon dye to observe Liver tissue structure and cellular morphology and the degree of fibrosis .Results Lipopolysaccharide and D-galactosamine combination resulted in ALT rise , hepatocyte degeneration and necrosis ,collagen fiber hyperplasia obviously . Conclusion D-galactosamine and Lipopolysaccharide combination could induce mice chronic hepatic injury modeling .