CAJ | 학술논문

目的：检测SPF级Rag2敲除小鼠的脏器重量，血生理生化等生物学特性指标和免疫细胞。方法分别选5、10周龄的Rag2敲除（ knockout ， KO）小鼠测定主要脏器重量，并测定主要血生理、生化指标。选取6周龄Rag2 KO小鼠，应用流式细胞仪检测细胞免疫（ T细胞-CD3＋、T细胞亚群-CD4＋、CD8＋）、体液免疫（ B细胞-CD19＋、B220＋）、NK细胞（ NK1.1＋）和粒细胞（ CD11b＋）进行检测。结果同性别NOD/SCID小鼠，10周小鼠脑、肺脏、脾脏、肝脏、心脏、双肾脏重、TBIL、WBC高于5周龄。同周龄的雌雄相比，雄性小鼠心脏、双肾脏、肝脏、脾脏重量、AST、ALP、A/G、GLU、PLT、PCT、WBC、LYM％高于雌性。 Rag2 KO小鼠无T细胞（0.36±0.15）％、CD4＋T细胞（0.21±0.06）％、CD8＋T 细胞（0.23±0.07）％、CD19＋B 细胞（0.28±0.04）％和 B220＋B 细胞（2.03±0.42）％）。 NK细胞比例为（24.13±3.62）％，粒细胞比例为（57.20±3.85）％。结论 Rag2 KO小鼠与C57BL/6J小鼠的主要生物学特性基本一致。周龄和性别因素对其脏器重量、血生理生化指标都有一定的影响。 Rag2 KO小鼠T、B淋巴细胞缺失。
목적：검측SPF급Rag2고제소서적장기중량，혈생리생화등생물학특성지표화면역세포。방법분별선5、10주령적Rag2고제（ knockout ， KO）소서측정주요장기중량，병측정주요혈생리、생화지표。선취6주령Rag2 KO소서，응용류식세포의검측세포면역（ T세포-CD3＋、T세포아군-CD4＋、CD8＋）、체액면역（ B세포-CD19＋、B220＋）、NK세포（ NK1.1＋）화립세포（ CD11b＋）진행검측。결과동성별NOD/SCID소서，10주소서뇌、폐장、비장、간장、심장、쌍신장중、TBIL、WBC고우5주령。동주령적자웅상비，웅성소서심장、쌍신장、간장、비장중량、AST、ALP、A/G、GLU、PLT、PCT、WBC、LYM％고우자성。 Rag2 KO소서무T세포（0.36±0.15）％、CD4＋T세포（0.21±0.06）％、CD8＋T 세포（0.23±0.07）％、CD19＋B 세포（0.28±0.04）％화 B220＋B 세포（2.03±0.42）％）。 NK세포비례위（24.13±3.62）％，립세포비례위（57.20±3.85）％。결론 Rag2 KO소서여C57BL/6J소서적주요생물학특성기본일치。주령화성별인소대기장기중량、혈생리생화지표도유일정적영향。 Rag2 KO소서T、B림파세포결실。
Objective To measure the organ weights , blood physiological and biochemical parameters , and immune function of Specific Pathogen Free ( SPF) Rag2 knockout ( KO) mice.Methods Rag2 knockout mice were selected at five and ten weeks , and the organ weights , blood physiological and biochemical parameters were observed .The percentages of CD3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in Rag2 KO mice at six week of age in terms of its T , B lymphocyte function and NK cell activity .Results Among the same sex group of NOD/SCID mice, the weights of brain, lung, spleen, liver, heart, kidney, and the levels of TBIL, WBC in 10 weeks of age are higher than 5 weeks.At the same age, the weights of heart, kidney, liver, spleen, and the levels of AST, ALP, A/G, GLU, PLT, PCT, WBC, and LYM%in male mice are higher than females.The Rag2 KO mice lacks T cells(0.36 ±0.15)%、CD4+T cells(0.21 ±0.06)%、CD8+T cells(0.23 ±0.07)%、CD19+B cells(0.28 ±0.04)%、B220+B cells(2.03 ±0.42)%).The percentage of NK cells is(24.13 ±3.62)%, and the percentage of granulocytes is (57.20 ±3.85)%.Conclusion The study suggests that the organ weights , blood physiological and biochemical parameters are affected by age and gender in Rag2 KO mice, which main biological characteristics are similar with C 57BL/6J mice.The Rag2 KO mice show the deficiency of T , B cells function .