CAJ | 학술논문

目的：测定NOD/SCID小鼠的主要脏器重量、血生理生化指标和免疫细胞比例。方法选取5周龄和10周龄的NOD/SCID小鼠，测定主要脏器重量和血液生理生化指标；选取6周龄的NOD/SCID小鼠，利用流式细胞仪检测其T细胞及其亚群（ T细胞-CD3＋、T细胞亚群-CD4＋T细胞、CD8＋T细胞）、B细胞（ CD19＋或B220＋）、NK细胞（NK1.1＋）和粒细胞（CD11b＋）。结果同性别NOD/SCID小鼠，不同周龄的双肾、肝脏、心脏和肺脏重量，血生理指标RBC、HGB、HCT、MCV、MCH和RDW，血生化指标TP、ALB、ALP、CHO和TBIL具有显著差异；同周龄的雌雄相比，血生理指标HCT，血生化指标GLOB、A/G、CHO、TG、TBIL和UN有显著差异。 NOD/SCID小鼠无T细胞（0.37±0.26）％、CD4＋T细胞（0.35±0.13）％、CD8＋T细胞（0.47±0.10）％、CD19＋B细胞（0.13±0.05）％、B220＋B细胞（1.20±0.44）％），有低水平NK细胞（6.90±0.82）％，粒细胞比例为（47.88±15.54）％。结论 NOD/SCID小鼠表现为为T、B、NK细胞联合免疫功能缺陷，周龄和性别对其脏器重量、血生理生化指标有一定影响。本研究的NOD/SCID小鼠品系的生理生化指标与国外生产的相同品系基本一致。
목적：측정NOD/SCID소서적주요장기중량、혈생리생화지표화면역세포비례。방법선취5주령화10주령적NOD/SCID소서，측정주요장기중량화혈액생리생화지표；선취6주령적NOD/SCID소서，이용류식세포의검측기T세포급기아군（ T세포-CD3＋、T세포아군-CD4＋T세포、CD8＋T세포）、B세포（ CD19＋혹B220＋）、NK세포（NK1.1＋）화립세포（CD11b＋）。결과동성별NOD/SCID소서，불동주령적쌍신、간장、심장화폐장중량，혈생리지표RBC、HGB、HCT、MCV、MCH화RDW，혈생화지표TP、ALB、ALP、CHO화TBIL구유현저차이；동주령적자웅상비，혈생리지표HCT，혈생화지표GLOB、A/G、CHO、TG、TBIL화UN유현저차이。 NOD/SCID소서무T세포（0.37±0.26）％、CD4＋T세포（0.35±0.13）％、CD8＋T세포（0.47±0.10）％、CD19＋B세포（0.13±0.05）％、B220＋B세포（1.20±0.44）％），유저수평NK세포（6.90±0.82）％，립세포비례위（47.88±15.54）％。결론 NOD/SCID소서표현위위T、B、NK세포연합면역공능결함，주령화성별대기장기중량、혈생리생화지표유일정영향。본연구적NOD/SCID소서품계적생리생화지표여국외생산적상동품계기본일치。
Objective To measure the organ weights , physiological and biochemical parameters and immune cells of NOD/SCID mice.Methods Mice at five and ten weeks of age were selected .The organ weights, blood physiological and biochemical parameters were observed .The percentages of CD 3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in NOD/SCID mice at six week of age in terms of its T , B lymphocyte function and NK cell activity.Results Among the same sex group of NOD/SCID mice, the weights of kidney, liver, heart and lung, and the blood physiological indexes of RBC , HGB, HCT, MCV, MCH, RDW, and the blood biochemical indexes of TP , ALB, ALP, CHO, TBIL show significant difference between 5 and 10 weeks.At the same age, HCT, GLOB, A/G, CHO, TG, TBIL and the UN are significantly different between male and female NOD /SCID mice.NOD/SCID mice lack T cells (0.37 ±0.26)%、CD4+T cells (0.35 ±0.13)%、CD8 +T cells (0.47 ±0.10)%、CD19+B cells (0.13 ± 0.05)%、B220+B cells (1.20 ±0.44)%.The percentage of NK cells is (6.90 ±0.82)%, and the percentage of granulocytes is (47.88 ±15.54)%.Conclusions The study indicates that NOD/SCID mice show the deficiency of T , B and NK cells function .The organ weights , blood physiological and biochemical parameters are affected by age and gender . The studied parameters of NOD/SCID mice are similar with the same strain in other countries .