CAJ | 학술논문

目的：测定SPF级BALB/cA-nu小鼠的主要脏器重量、血液生理生化值和免疫细胞比例。方法选取5周龄和10周龄的BALB/cA-nu小鼠，测定主要脏器重量，并检测血液生理生化指标。选取6周龄的BALB/cA-nu小鼠，应用流式细胞仪检测BALB/cA-nu小鼠的T细胞及其亚群（ T细胞-CD3＋、T细胞亚群-CD4＋T细胞、CD8＋T细胞）、B细胞（ CD19＋或B220＋）、NK细胞（ NK1.1＋）和粒细胞（ CD11b＋）。结果同周龄的BALB/cA-nu小鼠，雄鼠的体重、肝脏、双肾重量高于雌鼠（P ＜0.05），同时雄鼠的血生理指标HGB、MCH、HCT也明显高于雌鼠（P ＜0.05）。同性别的BALB/cA-nu小鼠，5周小鼠的肺脏、肝脏、双肾重量低于10周小鼠（P ＜0.05），5周小鼠的血生化指标TP、GLOB、CHO、TBIL、UN 也低于10周小鼠（ P ＜0.05）。 BALB/cA-nu 小鼠无T 细胞（0.18±0.06）％、CD4＋T细胞（0.26±0.08）％、CD8＋T细胞（0.13±0.04）％，B细胞的CD19＋B细胞的比例为（30.10±2.74）％、B220＋B细胞的比例为（30.55±2.77）％，NK细胞比例为（1.35±0.29）％，粒细胞比例为（47.90±5.48）％。结论 BALB/cA-nu小鼠表现为明显的T细胞免疫功能缺陷，周龄和性别因素对其脏器重量、血生理生化指标都有一定的影响。本研究的BALB/c小鼠品系的生理生化指标与国外生产的相同品系一致。
목적：측정SPF급BALB/cA-nu소서적주요장기중량、혈액생리생화치화면역세포비례。방법선취5주령화10주령적BALB/cA-nu소서，측정주요장기중량，병검측혈액생리생화지표。선취6주령적BALB/cA-nu소서，응용류식세포의검측BALB/cA-nu소서적T세포급기아군（ T세포-CD3＋、T세포아군-CD4＋T세포、CD8＋T세포）、B세포（ CD19＋혹B220＋）、NK세포（ NK1.1＋）화립세포（ CD11b＋）。결과동주령적BALB/cA-nu소서，웅서적체중、간장、쌍신중량고우자서（P ＜0.05），동시웅서적혈생리지표HGB、MCH、HCT야명현고우자서（P ＜0.05）。동성별적BALB/cA-nu소서，5주소서적폐장、간장、쌍신중량저우10주소서（P ＜0.05），5주소서적혈생화지표TP、GLOB、CHO、TBIL、UN 야저우10주소서（ P ＜0.05）。 BALB/cA-nu 소서무T 세포（0.18±0.06）％、CD4＋T세포（0.26±0.08）％、CD8＋T세포（0.13±0.04）％，B세포적CD19＋B세포적비례위（30.10±2.74）％、B220＋B세포적비례위（30.55±2.77）％，NK세포비례위（1.35±0.29）％，립세포비례위（47.90±5.48）％。결론 BALB/cA-nu소서표현위명현적T세포면역공능결함，주령화성별인소대기장기중량、혈생리생화지표도유일정적영향。본연구적BALB/c소서품계적생리생화지표여국외생산적상동품계일치。
Objective To measure the organ weights , blood physiological and biochemical parameters , and immune cells of BALB/cA-nu mice .Methods BALB/cA-nu mice at five and ten weeks of age were selected , and the organ weights , blood physiological and biochemical parameters were observed .The percentages of CD 3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were detected by FCM in BALB/cA-nu mice at six weeks of age in terms of its T, B lymphocyte cells and NK cell activity .Results At the same age, the weights of the body , liver, and kidney in male mice are significantly higher than females .The HGB、MCH、HCT in male mice are significantly higher than females (P <0.05).The weights of the lung, liver, and kidney, and the parameters of TP、GLOB、CHO、TBIL、UN are significantly lower at five weeks of age comparing with ten weeks (P <0.05).The BALB/cA-nu mice lacks T cells(0.18 ±0.06)%、CD4+T cells(0.26 ±0.08)%、CD8+T cells(0.13 ±0.04)%、The percentage of B cells is CD19+B cells (30.10 ±2.74)% and B220 +B cells(30.55 ±2.77)%.The percentage of NK cells is (1.35 ±0.29)%, and the percentage of granulocytes is (47.90 ±5.48)%.The BALB/cA-nu mice lack T cells and the group of cells .Conclusion The study suggests that the organ weights , blood physiological and biochemical parameters are affected by age and gender in BALB/cA-nu mice that lack T cells immune function .The studied parameters of BALB/cA-nu mice are similar with the same strain in other countries .