中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2014年
5期
31-34
,共4页
李曙芳%黄立群%岳娟%王永丽%王新钢%李炜宾%安全
李曙芳%黃立群%嶽娟%王永麗%王新鋼%李煒賓%安全
리서방%황립군%악연%왕영려%왕신강%리위빈%안전
亚低温%戊巴比妥钠%雄性小鼠%血液学
亞低溫%戊巴比妥鈉%雄性小鼠%血液學
아저온%무파비타납%웅성소서%혈액학
Mild hypothermia%Pentobarbital sodium%Mice%Hematology
目的:观察戊巴比妥钠诱导的亚低温对雄性BALB/C小鼠血液学指标的影响。方法将健康雄性BALB/C小鼠随机分为对照组(C)和亚低温组(M);亚低温组用戊巴比妥钠(100 mg/kg)腹腔注射诱导雄性BALB/C小鼠体温下降至28℃~30℃,维持4 h后自然复温,动物亚低温干预后2 h、24 h和72 h测定其肛温并取血测定血凝、电解质和血液细胞学指标;对照组给予等容体积的生理盐水,保持常温。结果本方法诱导的亚低温对雄性BALB/C小鼠体温和血凝三项与对照组相比差异无统计学意义( P﹥0.05), K+和Na+浓度高于对照组( P﹤0.01),外周血WBC低于对照组(P﹤0.01或P﹤0.05),白细胞分类比值发生变化,RBC、HGB、MCH、MCHC一过性降低( P﹤0.01或P﹤0.05)。结论戊巴比妥钠诱导的亚低温可对雄性BALB/C小鼠血液学指标产生影响。
目的:觀察戊巴比妥鈉誘導的亞低溫對雄性BALB/C小鼠血液學指標的影響。方法將健康雄性BALB/C小鼠隨機分為對照組(C)和亞低溫組(M);亞低溫組用戊巴比妥鈉(100 mg/kg)腹腔註射誘導雄性BALB/C小鼠體溫下降至28℃~30℃,維持4 h後自然複溫,動物亞低溫榦預後2 h、24 h和72 h測定其肛溫併取血測定血凝、電解質和血液細胞學指標;對照組給予等容體積的生理鹽水,保持常溫。結果本方法誘導的亞低溫對雄性BALB/C小鼠體溫和血凝三項與對照組相比差異無統計學意義( P﹥0.05), K+和Na+濃度高于對照組( P﹤0.01),外週血WBC低于對照組(P﹤0.01或P﹤0.05),白細胞分類比值髮生變化,RBC、HGB、MCH、MCHC一過性降低( P﹤0.01或P﹤0.05)。結論戊巴比妥鈉誘導的亞低溫可對雄性BALB/C小鼠血液學指標產生影響。
목적:관찰무파비타납유도적아저온대웅성BALB/C소서혈액학지표적영향。방법장건강웅성BALB/C소서수궤분위대조조(C)화아저온조(M);아저온조용무파비타납(100 mg/kg)복강주사유도웅성BALB/C소서체온하강지28℃~30℃,유지4 h후자연복온,동물아저온간예후2 h、24 h화72 h측정기항온병취혈측정혈응、전해질화혈액세포학지표;대조조급여등용체적적생리염수,보지상온。결과본방법유도적아저온대웅성BALB/C소서체온화혈응삼항여대조조상비차이무통계학의의( P﹥0.05), K+화Na+농도고우대조조( P﹤0.01),외주혈WBC저우대조조(P﹤0.01혹P﹤0.05),백세포분류비치발생변화,RBC、HGB、MCH、MCHC일과성강저( P﹤0.01혹P﹤0.05)。결론무파비타납유도적아저온가대웅성BALB/C소서혈액학지표산생영향。
Objective To observe the effects of mild hypothermia induced by pentobarbital sodium on hematology in male BALB/C mice.Method Healthy male BALB/C mice were divided randomly into two groups:control group ( C) and mild hypothermia group(M).The body temperature of the mild hypothermia group was maintained between 28℃ to 30℃( anal temperature ) for 4 hours induced by pentobarbital sodium injected intraperitoneally , then recover unaffected . Anal temperature, coagulation, electrolytes, and blood cell indexes were examined in 2, 24, 72 hours after treated by mild hypothermia;Control group was given equal volume of saline volume at constant temperature .Results The body temperature and coagulation in mild hypothermia group showed no significant difference compared with the control group ( P﹥0.05),but the concentration of K +and Na +in mild hypothermia group were higher than control group (P﹤0.01), the number of WBC in mild hypothermia group was lower than control group ( P﹤0.01或P﹤0.05 ) , and the RBC、HGB、MCH、MCHC in mild hypothermia group were lower than control group transiently (P﹤0.01或P﹤0.05).Conclusion Mild hypothermia induced by pentobarbital sodium affects some of hematological values in mice considerably .