CAJ | 학술논문

目的：研究人单核巨噬细胞株U937的90K/Mac-2BP 基因沉默后其mRNA和蛋白水平的表达及其对HIV-1感染单核巨噬细胞凋亡的影响。方法用人单核巨噬细胞株U937作为细胞模型，用R5嗜性的HIV-1全长感染性质粒包装并获得HIV-1病毒，待HIV-1感染U937细胞5 d后，细胞经PE-Annexin V，PerCP-7-AAD染色，用流式细胞仪检测细胞凋亡情况。采用基因沉默技术，将U937细胞中的90K/Mac-2BP基因沉默后，再经HIV-1感染，5 d后检测细胞凋亡情况。结果正常人单核巨噬细胞株 U937，经HIV-1感染后细胞凋亡率为（16.27±0.30）％，而90K/Mac-2BP 基因沉默后的U937细胞，经HIV-1感染后细胞凋亡率分别增加至（31.26±0.35）％、（25.76±0.30）％、（23.69±0.33）％，具有统计学差异（P＜0.05）。结论90K/Mac-2BP的表达水平能调节HIV-1感染的单核巨噬细胞凋亡。
목적：연구인단핵거서세포주U937적90K/Mac-2BP 기인침묵후기mRNA화단백수평적표체급기대HIV-1감염단핵거서세포조망적영향。방법용인단핵거서세포주U937작위세포모형，용R5기성적HIV-1전장감염성질립포장병획득HIV-1병독，대HIV-1감염U937세포5 d후，세포경PE-Annexin V，PerCP-7-AAD염색，용류식세포의검측세포조망정황。채용기인침묵기술，장U937세포중적90K/Mac-2BP기인침묵후，재경HIV-1감염，5 d후검측세포조망정황。결과정상인단핵거서세포주 U937，경HIV-1감염후세포조망솔위（16.27±0.30）％，이90K/Mac-2BP 기인침묵후적U937세포，경HIV-1감염후세포조망솔분별증가지（31.26±0.35）％、（25.76±0.30）％、（23.69±0.33）％，구유통계학차이（P＜0.05）。결론90K/Mac-2BP적표체수평능조절HIV-1감염적단핵거서세포조망。
Objective To investigate the effect of cell death by HIV-1 infection on gene 90K/Mac-2BP by RNA interference (RNAi) in U937 cell line.Methods We used human monocyte-macrophage cell line U937 as the cell model.Cells were infected by HIV-1 ( R5-tropic) 5 days, and then stained by PE-Annexin V and PerCP-7-AAD.90K/Mac-2BP in U937 cell line was knocked down , and these cells were infected by HIV-1 for 5 days.Then, cells were stained by PE-AnnexinV and PerCP-7-AAD.Apoptosis were examined upon flow cytometry .Results The percentages of Annexin V+cells without 90K/Mac-2BP knock-down were (16.27 ±0.30)% by HIV-1 infection.The percentages of them with 90K/Mac-2BP knock-down were (31.26 ±0.35)%, (25.76 ±0.30)%, (23.69 ±0.33)% respectively.The increase of cell apoptosis rate for HIV-1-infected U937 cells by 90K/Mac-2BP siRNA transfection was significantly greater than that for HIV-1-infected untreated cells (P﹤0.01).Conclusion The apoptosis of HIV-1-infected U937 cells was regulated by the expression of 90K/Mac-2BP.