CAJ | 학술논문

目的：研究复方红景天制剂对高正加速度（ positive acceleration，＋Gz）应激大鼠血清皮质酮及心肌组织糖皮质激素受体的影响。方法72只雄性SD大鼠随机分为6组，分别为空白对照组、应激对照组、＋10 Gz应激组以及药物低、中、高剂量组，每组12只。各组均灌胃给药14 d，低、中、高剂量组大鼠每天分别按0.75 g/kg、1.5 g/kg和3.0 g/kg给予复方红景天中药制剂，其余3组动物给等量的生理盐水。第15天进行＋10 Gz离心机暴露，ELISA方法测定各组动物血清皮质酮浓度，免疫印迹法分析各组动物心肌组织糖皮质激素受体表达变化。结果与空白对照组和应激对照组相比，＋10 Gz应激组大鼠血清皮质酮浓度明显升高，而心肌组织糖皮质激素受体表达水平明显降低（ P ＜0.01，P ＜0.05）。复方红景天制剂预处理可以降低＋10 Gz应激大鼠血清皮质酮浓度，上调心肌组织糖皮质激素受体表达水平，药物中、高剂量组大鼠血清皮质酮浓度明显低于＋10 Gz应激组（P ＜0.05），而心肌组织糖皮质激素受体表达水平明显高于＋10 Gz应激组（P ＜0.01）。结论复方红景天制剂可以调节＋Gz应激大鼠血清糖皮质激素浓度及其心肌受体表达水平，可能与该制剂对持续正加速度致心肌损伤的防护作用有关。
목적：연구복방홍경천제제대고정가속도（ positive acceleration，＋Gz）응격대서혈청피질동급심기조직당피질격소수체적영향。방법72지웅성SD대서수궤분위6조，분별위공백대조조、응격대조조、＋10 Gz응격조이급약물저、중、고제량조，매조12지。각조균관위급약14 d，저、중、고제량조대서매천분별안0.75 g/kg、1.5 g/kg화3.0 g/kg급여복방홍경천중약제제，기여3조동물급등량적생리염수。제15천진행＋10 Gz리심궤폭로，ELISA방법측정각조동물혈청피질동농도，면역인적법분석각조동물심기조직당피질격소수체표체변화。결과여공백대조조화응격대조조상비，＋10 Gz응격조대서혈청피질동농도명현승고，이심기조직당피질격소수체표체수평명현강저（ P ＜0.01，P ＜0.05）。복방홍경천제제예처리가이강저＋10 Gz응격대서혈청피질동농도，상조심기조직당피질격소수체표체수평，약물중、고제량조대서혈청피질동농도명현저우＋10 Gz응격조（P ＜0.05），이심기조직당피질격소수체표체수평명현고우＋10 Gz응격조（P ＜0.01）。결론복방홍경천제제가이조절＋Gz응격대서혈청당피질격소농도급기심기수체표체수평，가능여해제제대지속정가속도치심기손상적방호작용유관。
Objective To study the effect of rhodiola crenulata compound on serum corticosterone and myocardial glucocorticoid receptor ( GR) in rats exposed high sustained +Gz.Methods Seventy-two healthy SD rats were randomly divided into six groups: blank control group, stress control group, +10 Gz stress group and low, medium, high dose drug group, with twelve rats in each.20 mL/kg menstruum was fed to each rat once per day for 14 days.Low, medium and high dose drug group were fed with rhodiola crenulata compound at doses of 0.75 g/kg, 1.5 g/kg and 3.0 g/kg respectively, the other three groups were fed with equal volume saline.Rats were exposed to high +Gz in 15th day.The concentration of corticosterone in the serum of each group rats was detected with ELISA. Western blot was used to detect the expressions of GR in the myocardium of each group rats .Results The content of corticosterone was significantly elevated in +10Gz stress group, while the expression of GR in the myocardium was markedly declined (P <0.01,P <0.05).However, compound preconditioning could decrease concentration of corticosterone in the serum and enhance the expression of GR in the myocardium from rats after +10 Gz exposure. The corticosterone concentration of medium and high dose drug groups was significantly lower and the level of GR expression in the myocardium was significantly higher than that of +10 Gz stress group ( P <0.05 , P <0.01 ) . Conclusion Rhodiola crenulata compound preconditioning could regulate the concentration of corticosterone in the serum and the level of GR expression in the myocardium of the rats exposed +10 Gz, which may be related with its protective effect on high sustained +Gz-induced injury of myocardium.