南昌大学学报(医学版)
南昌大學學報(醫學版)
남창대학학보(의학판)
ACTA ACADEMIAE MEDICINAE JIANGXI
2014年
4期
14-17
,共4页
陈文龙%周从阳%舒维%谢姝
陳文龍%週從暘%舒維%謝姝
진문룡%주종양%서유%사주
乌司他丁%百草枯%中毒%急性肺损伤%动物,实验%大鼠
烏司他丁%百草枯%中毒%急性肺損傷%動物,實驗%大鼠
오사타정%백초고%중독%급성폐손상%동물,실험%대서
ulinastatin%paraquat%poisoning%acute lung injury%animal,laboratory%rats
目的:探讨不同剂量乌司他丁(UTI)对大鼠百草枯(PQ)急性肺损伤(ALI)的保护作用。方法将50只SD大鼠按随机数字表法分为5组:阴性对照组(NC组),阳性对照组(PC组),UTI治疗小剂量组(SD组)、中剂量组(MD组)、大剂量组(LD组),每组10只。阳性对照组与 UTI治疗组大鼠采用百草枯一次性灌胃染毒法(80 mg· kg-1)制作PQ中毒肺损伤模型,阴性对照组采用等量生理盐水灌胃。灌胃30 min后,SD组、MD组、LD组分别腹腔注射UTI 100、300、600 U·kg-1,NC组、PC组注射等量生理盐水。腹腔注射24 h后观察各组大鼠一般情况;测定支气管肺泡灌洗液(BALF)白细胞总数及中性粒细胞百分比、蛋白含量;光镜下观察肺组织病理学变化,进行肺损伤病理评分。结果 PC组BALF白细胞总数、中性粒细胞百分比、蛋白含量及肺损伤病理评分均明显高于 NC组(P<0.05);SD、MD、LD组BALF白细胞总数、中性粒细胞百分比、蛋白含量及肺损伤病理评分较 PC组均明显减少(P<0.05),且随着 UTI用量增加,逐渐降低(P<0.05)。结论乌司他丁对大鼠百草枯急性肺损伤具有保护作用,且随着剂量增加保护作用逐渐增强。
目的:探討不同劑量烏司他丁(UTI)對大鼠百草枯(PQ)急性肺損傷(ALI)的保護作用。方法將50隻SD大鼠按隨機數字錶法分為5組:陰性對照組(NC組),暘性對照組(PC組),UTI治療小劑量組(SD組)、中劑量組(MD組)、大劑量組(LD組),每組10隻。暘性對照組與 UTI治療組大鼠採用百草枯一次性灌胃染毒法(80 mg· kg-1)製作PQ中毒肺損傷模型,陰性對照組採用等量生理鹽水灌胃。灌胃30 min後,SD組、MD組、LD組分彆腹腔註射UTI 100、300、600 U·kg-1,NC組、PC組註射等量生理鹽水。腹腔註射24 h後觀察各組大鼠一般情況;測定支氣管肺泡灌洗液(BALF)白細胞總數及中性粒細胞百分比、蛋白含量;光鏡下觀察肺組織病理學變化,進行肺損傷病理評分。結果 PC組BALF白細胞總數、中性粒細胞百分比、蛋白含量及肺損傷病理評分均明顯高于 NC組(P<0.05);SD、MD、LD組BALF白細胞總數、中性粒細胞百分比、蛋白含量及肺損傷病理評分較 PC組均明顯減少(P<0.05),且隨著 UTI用量增加,逐漸降低(P<0.05)。結論烏司他丁對大鼠百草枯急性肺損傷具有保護作用,且隨著劑量增加保護作用逐漸增彊。
목적:탐토불동제량오사타정(UTI)대대서백초고(PQ)급성폐손상(ALI)적보호작용。방법장50지SD대서안수궤수자표법분위5조:음성대조조(NC조),양성대조조(PC조),UTI치료소제량조(SD조)、중제량조(MD조)、대제량조(LD조),매조10지。양성대조조여 UTI치료조대서채용백초고일차성관위염독법(80 mg· kg-1)제작PQ중독폐손상모형,음성대조조채용등량생리염수관위。관위30 min후,SD조、MD조、LD조분별복강주사UTI 100、300、600 U·kg-1,NC조、PC조주사등량생리염수。복강주사24 h후관찰각조대서일반정황;측정지기관폐포관세액(BALF)백세포총수급중성립세포백분비、단백함량;광경하관찰폐조직병이학변화,진행폐손상병리평분。결과 PC조BALF백세포총수、중성립세포백분비、단백함량급폐손상병리평분균명현고우 NC조(P<0.05);SD、MD、LD조BALF백세포총수、중성립세포백분비、단백함량급폐손상병리평분교 PC조균명현감소(P<0.05),차수착 UTI용량증가,축점강저(P<0.05)。결론오사타정대대서백초고급성폐손상구유보호작용,차수착제량증가보호작용축점증강。
Objective To investigate the effects of different doses of ulinastatin(UTI)on pa-raquat(PQ)-induced acute lung inj ury(ALI)in rats.Methods Fifty SD rats were randomly divid-ed into five groups:normal control group(NC group),positive control group(PQ group),low dose UTI treatment group(SD group),middle dose UTI treatment group(MD group)and high dose UTI treatment group(LD group),with 10 rats in each group.ALI was induced by paraquat gavage (80 mg·kg-1 )in NC group and UTI treatment groups.The NC group was given the same vol-ume of saline.In addition,rats in SD,MD and LD groups were intraperitoneally inj ected with UTI at doses of 100,300 and 600 U·kg-1 30 minutes after gavage administration,respectively.Rats in NC and PC groups were inj ected with the same volume of saline.The general conditions of rats were observed 24 hours after intraperitoneal injection.Moreover,the number of white blood cells, percentage of neutrophilic granulocytes and content of protein in bronchoalveolar lavage fluid (BALF)were determined,and pathologic changes in the lung were observed under the light mi-croscope.Results Compared with NC group,the number of white blood cells,percentage of neu-trophilic granulocytes,protein content and pathological score were significantly increased in PQ group(P<0.05).Compared with PC group,these parameters were significantly decreased by UTI treatment in a dose-dependent manner(P<0.05).Conclusion UTI treatment can protect against PQ-induced ALI in a dose-dependent manner in rats.