CAJ | 학술논문

目的观察褪黑素(MT)对睡眠剥夺(SD)大鼠空间学习记忆能力的影响.方法小平台水环境法制备SD大鼠模型.高效液相法检测不同时程SD大鼠松果体内MT含量.Morris水迷宫方法检测大鼠的空间学习记忆能力,每天训练前30 min腹腔注射MT.结果松果体内MT含量与SD时间有关,随着SD时间的延长,MT含量逐渐下降,睡眠恢复后,MT含量明显增加.与对照组大鼠比较,SD大鼠的逃避潜伏期明显延长,跨越原平台象限次数减少；在SD期间补充外源性MT后,上述指标与对照组比较差异无统计学意义(P＞0.05).结论 SD引起的空间学习记忆能力下降与松果体内MT分泌减少有关,补充外源性MT能预防这种因睡眠不足导致的学习记忆能力下降.
목적 관찰퇴흑소(MT)대수면박탈(SD)대서공간학습기억능력적영향.방법 소평태수배경법제비SD대서모형.고효액상법검측불동시정SD대서송과체내MT함량.Morris수미궁방법검측대서적공간학습기억능력,매천훈련전30 min복강주사MT.결과 송과체내MT함량여SD시간유관,수착SD시간적연장,MT함량축점하강,수면회복후,MT함량명현증가.여대조조대서비교,SD대서적도피잠복기명현연장,과월원평태상한차수감소；재SD기간보충외원성MT후,상술지표여대조조비교차이무통계학의의(P＞0.05).결론 SD인기적공간학습기억능력하강여송과체내MT분비감소유관,보충외원성MT능예방저충인수면불족도치적학습기억능력하강.
Objective To observe the effect of melatonin (MT) on the spatial learning and memory of sleep deprived (SD) rats.Methods The deprivation of sleep was accomplished by maintaining rats on a small platform surrounded by water.High performance liquid chromatography was used to test the MT content in the pineal gland.The spatial learning and memory ability of the rats was tested with Morris water maze.Rats were intraplantarly injected with MT (15 mg/kg),then Morris water maze test was examined 30 min later.Results In the present study,high performance liquid chromatography showed that the MT content in the pineal gland was significantly reduced with the extention of SD,and it was markedly increased after sleep recovery.Compared with control group,the escape latency was prolonged and the numbers of crossing hidden platform were decreased of SD group; and there was no significant difference between control group and SD + MT group.Conclusion The results demonstrate that the reduction of spatial learning and memory ability during SD is related to decreased MT in the pineal gland,and it can be prevented by exogenous MT supply.