CAJ | 학술논문

为提高米渣酶解制备抗氧化肽的反应效率，采用超声预处理米渣。以酶解液对1,1-二苯基-2-苦基苯肼（DPPH）自由基的清除率作为评价指标，研究超声功率、超声时间、超声温度对米渣酶解产物抗氧化活性的影响。通过单因素和正交试验，得出超声预处理最佳工艺条件为超声功率150 W，超声时间15 min，超声温度50℃。在最佳条件下，酶解液对DPPH自由基的清除率为75.41%，抗氧化肽的得率为35.55%。与未经超声预处理比较，抗氧化肽得率提高了39.86%，酶解液对DPPH自由基清除率、·OH清除力和还原力吸光度的增长率分别为54.06%，64.87%和82.27%。
위제고미사매해제비항양화태적반응효솔，채용초성예처리미사。이매해액대1,1-이분기-2-고기분정（DPPH）자유기적청제솔작위평개지표，연구초성공솔、초성시간、초성온도대미사매해산물항양화활성적영향。통과단인소화정교시험，득출초성예처리최가공예조건위초성공솔150 W，초성시간15 min，초성온도50℃。재최가조건하，매해액대DPPH자유기적청제솔위75.41%，항양화태적득솔위35.55%。여미경초성예처리비교，항양화태득솔제고료39.86%，매해액대DPPH자유기청제솔、·OH청제력화환원력흡광도적증장솔분별위54.06%，64.87%화82.27%。
In order to enhance enzymatic hydrolysis in preparing antioxidant peptide from rice residue, rice residue is pretreated by ultrasonic. Based on the scavenging rate of hydrolysate to the DPPH radical, the effects of ultrasonic power, time and temperature on ultrasonic pretreatment efficacy are studied. The optimum conditions by single-factor experiment and orthogonal experiment are as follows: ultrasonic power 150 W, pretreatment time 15 min and pretreatment temperature 50℃. Under the optimum conditions, scavenging rate of hydrolysate to the DPPH radical and antioxidant peptide output are 75.41%and 35.55%, respectively. Compared with the traditional method, antioxidant peptide output improved 39.86%, the scavenging rate for DPPH· free radical, scavenging rate for ·OH and the ferric reducing ability of power of the antioxidant peptides after ultrasonic pretreatment are increased respectively by 54.06%, 64.87%and 82.27%.