CAJ | 학술논문

目的：研究参芪扶正注射液体内外对肝癌H22细胞的抑制作用及其机制。方法 MTT法检测参芪扶正注射液体外对H22细胞增殖的影响；利用H22小鼠模型进行体内抗肿瘤作用试验，计算抑瘤率；酶联免疫ELISA法测荷瘤小鼠血清IFN-γ含量；双抗体夹心ABC-ELSA法测荷瘤小鼠血清TNF-α的含量。结果参芪扶正注射液体内外均能抑制H22细胞的生长，具有浓度和时间依赖性；促进荷瘤小鼠血清中IFN-γ和TNF-α的表达。结论参芪扶正注射液体内外均能抑制肝癌H22的生长，其抗肿瘤作用与调节荷瘤小鼠的免疫功能有关。
목적：연구삼기부정주사액체내외대간암H22세포적억제작용급기궤제。방법 MTT법검측삼기부정주사액체외대H22세포증식적영향；이용H22소서모형진행체내항종류작용시험，계산억류솔；매련면역ELISA법측하류소서혈청IFN-γ함량；쌍항체협심ABC-ELSA법측하류소서혈청TNF-α적함량。결과삼기부정주사액체내외균능억제H22세포적생장，구유농도화시간의뢰성；촉진하류소서혈청중IFN-γ화TNF-α적표체。결론삼기부정주사액체내외균능억제간암H22적생장，기항종류작용여조절하류소서적면역공능유관。
Objective To study the inhibition effect and mechanism on H22 of Shenqifuzheng injection. Methods MTT was performed to observe the proliferation of H22 cell in vitro;then anticancer experiment in vivo was done by H22 mice model,inhibitory rate was calculated;the serum content of IFN-γwas measured by ELISA assay;the content of TNF-α by enzyme-linked immunosorbent assay. Results Shenqifuzheng injection inhibited the proliferation of H22 cells in a concentration and timedependent manner,promoted the expression of IFN-γ and TNF-α in the serum of tumor-bearing mice. Conclusion Shenqifuzheng injection can inhibit the growth of H22 in vitro and in vivo,the an-ti-tumor effect related with the immune function of tumor-bearing mice improved.