CAJ | 학술논문

[目的]该研究旨在研究沙门氏菌多重耐药性与Ⅰ型整合子的流行情况。[方法]该研究从山东省鸡场分离沙门氏菌，根据Kauffmann-White方法测定其血清型，采用微量稀释法测定了沙门氏菌对19种常用抗菌药物的MIC值，并且采用 PCR技术测定了Ⅰ型整合子及其携带的耐药基因盒。[结果]本次分离得到311株沙门氏菌，包括印地安纳沙门氏菌（133株）和肠炎沙门氏菌（178株）2种血清型；药物敏感性试验表明：分离菌株对磺胺嘧啶、磺胺甲恶唑、萘啶酸、氨苄西林、四环素、多西环素和甲氧苄啶普遍耐药，菌株多重耐药率为91.0%(283/311)，99%的分离株对阿米卡星、多黏菌素敏感；PCR测定Ⅰ型整合子结果：Ⅰ型整合子的检出率为65.0%(202/311)，其中多重耐药表型菌株Ⅰ型整合子阳性率为92.6%，202株整合子阳性菌株中有6株的携带基因盒dfr17-aadA5。[结论]Ⅰ型整合子普遍存在于沙门氏菌中，并且与沙门氏菌的多重耐药性密切相关。
[목적]해연구지재연구사문씨균다중내약성여Ⅰ형정합자적류행정황。[방법]해연구종산동성계장분리사문씨균，근거Kauffmann-White방법측정기혈청형，채용미량희석법측정료사문씨균대19충상용항균약물적MIC치，병차채용 PCR기술측정료Ⅰ형정합자급기휴대적내약기인합。[결과]본차분리득도311주사문씨균，포괄인지안납사문씨균（133주）화장염사문씨균（178주）2충혈청형；약물민감성시험표명：분리균주대광알밀정、광알갑악서、내정산、안변서림、사배소、다서배소화갑양변정보편내약，균주다중내약솔위91.0%(283/311)，99%적분리주대아미잡성、다점균소민감；PCR측정Ⅰ형정합자결과：Ⅰ형정합자적검출솔위65.0%(202/311)，기중다중내약표형균주Ⅰ형정합자양성솔위92.6%，202주정합자양성균주중유6주적휴대기인합dfr17-aadA5。[결론]Ⅰ형정합자보편존재우사문씨균중，병차여사문씨균적다중내약성밀절상관。
Objective This study aimed to investigate the multidrug resistance and prevalence of class I integrons in Salmonel a. [Method] Salmonel a strains were iso-lated from chicken farms in Shandong Province. Kauffmann-White classification method was employed to analyze the serotypes of Salmonel a strains. Minimum in-hibition concentration (MIC) of Salmonel a strains against 19 common antimicrobial drugs was analyzed determined with microdilution method. The class I integrons and carried drug resistance gene cassettes were detected by PCR. [Result] A total of 311 Salmonel a strains were isolated and classified into two serotypes, including 133 Salmonel a Indiana strains and 178 Salmonel a Enteritidis strains. Drug sensitivity test showed that the isolated Salmonel a strains were general y resistant to sulfadi-azine, sulfamethoxazole, nalidixic acid, ampicil in, tetracycline, doxycycline and trimethoprim, with a multidrug resistance rate of 91.0% (283/311); 99% strains were sensitive to amikacin and colistin. PCR assay indicated that the detection rate of class I integrons was 65.0% (202/311); the positive rate of class I integrons in Salmonel a strains with multidrug resistance was 92.6%; among 202 positive strains, six strains carried gene cassette dfr17-aadA5. [Conclusion] According to the above results, class I integrons exist general y in Salmonel a and are closely associated with the multidrug resistance of Salmonel a strains.