CAJ | 학술논문

该研究采用水培试验方法，用不同浓度的Cd溶液培养大豆幼苗14天，研究了 Cd在大豆幼苗叶片中的累积、亚细胞分配与定位及幼苗的生长状况。结果表明：低浓度的 Cd对大豆幼苗的生长具有刺激效应，但高浓度的Cd胁迫能抑制其生长；叶中 Cd随着溶液中Cd浓度的增加而显著增加；在高浓度 Cd胁迫下，绝大部分 Cd束缚在细胞壁和可溶性成分中，少部分储存在细胞核、叶绿体、线粒体等细胞器中；在细胞壁、叶绿体、细胞核和液泡中可以观察到黑色 Cd颗粒沉淀；细胞间隙扩大，亚细胞结构受损尤其是叶绿体；细胞壁是叶细胞保护内部细胞器的第一屏障，细胞壁和细胞可溶性成分是储存 Cd的主要部位。
해연구채용수배시험방법，용불동농도적Cd용액배양대두유묘14천，연구료 Cd재대두유묘협편중적루적、아세포분배여정위급유묘적생장상황。결과표명：저농도적 Cd대대두유묘적생장구유자격효응，단고농도적Cd협박능억제기생장；협중 Cd수착용액중Cd농도적증가이현저증가；재고농도 Cd협박하，절대부분 Cd속박재세포벽화가용성성분중，소부분저존재세포핵、협록체、선립체등세포기중；재세포벽、협록체、세포핵화액포중가이관찰도흑색 Cd과립침정；세포간극확대，아세포결구수손우기시협록체；세포벽시협세포보호내부세포기적제일병장，세포벽화세포가용성성분시저존 Cd적주요부위。
In order to investigate Cd accumulation, subcellular distribution, and local-ization in soybean seedlings leaves, soybean seedlings were cultivated in solution containing different concentrations of Cd. The results showed that most Cd associ-ated with the cellwal s and soluble fractions, and a minor part of Cd presented in mitochondria fractions, nuclear and chloroplast fractions, especial y exposure to high Cd concentrations. Under 20.00 mg/L Cd stress, Cd subcellular distribution fol owed a sequence as: soluble fractions (55.00%)>cellwal s (30.0%)>mitochondria fractions (8.21%)>nuclear and chloroplast fractions (6.79%). Deposited Cd black particles were observed in cellwal s, chloroplasts, nuclei, and vacuoles through electrical mi-croscope slice. This fact indicated that the cellwal s of soybean leaves were the first protecting organel es from Cd toxicity, and the cellwal s and soluble fractions were the main place for Cd storage. Due to Cd accumulated in the organel es, the intercellular space was enlarged and the subcellular structure was damaged, espe-cial y for the chloroplasts.