CAJ | 학술논문

为检测水稻纹枯病抗性基因位点，采用牙签接种法对籼稻9311和粳稻日本晴以及由它们构建的119个染色体单片段置换系进行纹枯病抗性鉴定，并使用该群体的分子连锁图谱进行数量性状座位（QTL）分析。共检测到3个纹枯病相关 QTL（qsb8-1， qsb8-2和 qsb8-3），分别位于第8染色体相邻标记 RM3262、RM5485和 RM3496附近，所在遗传区间分别为81.7cM~91.7cM、91.7cM~108.1cM和108.1cM~119.6cM。其中 qsb8-2的加性效应为负值，表明感病亲本携带的该片段可以增强纹枯病抗性；qsb8-1和qsb8-3的加性效应为正值，表明感病亲本携带的该片段减弱了纹枯病抗性。
위검측수도문고병항성기인위점，채용아첨접충법대선도9311화갱도일본청이급유타문구건적119개염색체단편단치환계진행문고병항성감정，병사용해군체적분자련쇄도보진행수량성상좌위（QTL）분석。공검측도3개문고병상관 QTL（qsb8-1， qsb8-2화 qsb8-3），분별위우제8염색체상린표기 RM3262、RM5485화 RM3496부근，소재유전구간분별위81.7cM~91.7cM、91.7cM~108.1cM화108.1cM~119.6cM。기중 qsb8-2적가성효응위부치，표명감병친본휴대적해편단가이증강문고병항성；qsb8-1화qsb8-3적가성효응위정치，표명감병친본휴대적해편단감약료문고병항성。
In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with sheath blight resis-tance in rice with toothpick inoculation method. A total of three sheath blight resis-tance-associated QTLs (qsb8-1, qsb8-2 and qsb8-3) were identified, which were lo-cated on adjacent molecular markers RM3262, RM5485 and RM3496 of chromo-some 8; the genetic interval was 81.7cM-91.7cM, 91.7cM-108.1cM and 108.1cM-119.6cM, respectively. The additive effect of qsb8-2 was negative, indicating that sheath blight resistance of susceptible parent harboring qsb8-2 fragment was en-hanced; additive effects of qsb8-1 and qsb8-3 were positive, indicating that sheath blight resistance of susceptible parent harboring qsb8-1 and qsb8-3 fragments was reduced.