国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
6期
992-995
,共4页
糖尿病视网膜病变%血糖波动%DNA损伤%单细胞凝胶电泳
糖尿病視網膜病變%血糖波動%DNA損傷%單細胞凝膠電泳
당뇨병시망막병변%혈당파동%DNA손상%단세포응효전영
diabetic retinopathy%blood glucose fluctuation%DNA damage%single cell gel electrophoresis
目的：观察糖尿病大鼠视网膜组织DNA损伤的程度,探讨血糖波动对其的影响,为研究糖尿病视网膜病变( diabetic retinopathy,DR)的发病机制提供新的思路。<br> 方法：SD大鼠随机分为正常对照组( NC )、正常波动组( NF)、糖尿病模型组( DM)和糖尿病波动组( DF)。腹腔注射STZ诱导糖尿病,NF和DF两组大鼠每天三次腹腔注射葡萄糖造成血糖波动,第8 wk取视网膜,采用单细胞凝胶电泳( SCGE)技术检测DNA损伤程度。<br> 结果：NF和DF两组大鼠血糖变化明显而且规律,波动曲线稳定,评价血糖稳定性的各项指标较NC组均有明显的增高,DF组升高更显著,差异有统计学意义(P<0.01)。SCGE显示NF,DM和DF三组细胞均有不同程度的DNA损伤,彗星尾长( TL )、尾部 DNA 含量( TDNA%)、尾矩(TM)和Olive尾矩(OTM)四指标均高于NC组,多组及两组间比较差异均有统计学意义(P<0.01)。<br> 结论：糖尿病大鼠视网膜组织存在DNA损伤,血糖波动可加重损伤,表明高血糖和血糖波动可能参与了视网膜组织DNA损伤的机制,可能是DR发生的早期事件之一,在DR的发生发展中起了重要的作用。
目的：觀察糖尿病大鼠視網膜組織DNA損傷的程度,探討血糖波動對其的影響,為研究糖尿病視網膜病變( diabetic retinopathy,DR)的髮病機製提供新的思路。<br> 方法：SD大鼠隨機分為正常對照組( NC )、正常波動組( NF)、糖尿病模型組( DM)和糖尿病波動組( DF)。腹腔註射STZ誘導糖尿病,NF和DF兩組大鼠每天三次腹腔註射葡萄糖造成血糖波動,第8 wk取視網膜,採用單細胞凝膠電泳( SCGE)技術檢測DNA損傷程度。<br> 結果：NF和DF兩組大鼠血糖變化明顯而且規律,波動麯線穩定,評價血糖穩定性的各項指標較NC組均有明顯的增高,DF組升高更顯著,差異有統計學意義(P<0.01)。SCGE顯示NF,DM和DF三組細胞均有不同程度的DNA損傷,彗星尾長( TL )、尾部 DNA 含量( TDNA%)、尾矩(TM)和Olive尾矩(OTM)四指標均高于NC組,多組及兩組間比較差異均有統計學意義(P<0.01)。<br> 結論：糖尿病大鼠視網膜組織存在DNA損傷,血糖波動可加重損傷,錶明高血糖和血糖波動可能參與瞭視網膜組織DNA損傷的機製,可能是DR髮生的早期事件之一,在DR的髮生髮展中起瞭重要的作用。
목적：관찰당뇨병대서시망막조직DNA손상적정도,탐토혈당파동대기적영향,위연구당뇨병시망막병변( diabetic retinopathy,DR)적발병궤제제공신적사로。<br> 방법：SD대서수궤분위정상대조조( NC )、정상파동조( NF)、당뇨병모형조( DM)화당뇨병파동조( DF)。복강주사STZ유도당뇨병,NF화DF량조대서매천삼차복강주사포도당조성혈당파동,제8 wk취시망막,채용단세포응효전영( SCGE)기술검측DNA손상정도。<br> 결과：NF화DF량조대서혈당변화명현이차규률,파동곡선은정,평개혈당은정성적각항지표교NC조균유명현적증고,DF조승고경현저,차이유통계학의의(P<0.01)。SCGE현시NF,DM화DF삼조세포균유불동정도적DNA손상,혜성미장( TL )、미부 DNA 함량( TDNA%)、미구(TM)화Olive미구(OTM)사지표균고우NC조,다조급량조간비교차이균유통계학의의(P<0.01)。<br> 결론：당뇨병대서시망막조직존재DNA손상,혈당파동가가중손상,표명고혈당화혈당파동가능삼여료시망막조직DNA손상적궤제,가능시DR발생적조기사건지일,재DR적발생발전중기료중요적작용。
AIM:To observe the situation of rat retinal tissue DNA damage at early diabetic period, discuss the role of the blood glucose fluctuations, and provide a new method for studying the pathogenesis of diabetic retinopathy ( DR) . <br> METHODS: SD rats were randomly divided into four groups:normal control group (NC), normal fluctuation group ( NF ) , diabetes group ( DM ) and diabetes fluctuation group ( DF ) . Diabetic models were established through intraperitoneal injection of STZ. A certain amount of glucose was injected in the rats of group NF and DF in an intraperitoneal mode three times a day after the model was established, thereby causing blood glucose fluctuations. Rats were killed and the retinal tissues were taken in the 8th week. Single cell gel electrophoresis ( SCGE ) technique was adopted for detecting DNA injury extent in the retina tissue. <br> RESULTS:Groups NF and DF showed significant and regular fluctuations. The curve of blood glucose fluctuations was relatively stable. All values of MBG, SDBG, LAGE and M were significantly increased compared with group NC. Group DF was increased more significantly. It was statistically significant (P<0. 01). SCGE showed that there were DNA damages in different levels in the cells of group NF, DM and DF. Indicators of cells such as TL, TDNA %, TM, OTM were higher than that in group NC. It was statistically significant ( P<0-01). The comparison difference between two groups was also significant (P<0. 01). <br> CONCLUSION: Rat retinal tissues have DNA injury during early diabetic period. DNA injury is gradually aggravated with blood glucose fluctuation. It indicates that high blood glucose and blood glucose fluctuation are involved in the mechanism of cell DNA injury, and they may be one of DR early event, have played a certain role in the incidence of DR.
