CAJ | 학술논문

目的：研究短时间吸入七氟醚对发育中大鼠海马神经元凋亡的影响。方法78只1个月龄 SD大鼠按随机数字表法随机分为3组，空气对照组（Sa，26只），载气对照组（Sc，26只），3％七氟醚吸入2 h 组（S2，26只），建立七氟醚吸入模型，吸入结束后12 h 用旷场实验和 T 迷宫进行神经行为学测试，同时断头取海马组织，苏木素-伊红染色观察海马神经元形态学改变，用流式细胞仪检测凋亡，激光共聚焦显微镜测神经元细胞内游离钙离子浓度。结果 S2组海马凋亡神经元数目以及神经元内游离钙离子相对荧光像素值均高于对照组，差异有统计学意义（P<0.05）；但神经行为学检测无明显差别。结论七氟醚可诱导幼龄大鼠海马神经元凋亡，可能通过升高细胞内钙离子浓度而触发凋亡。但单次、短时间吸入（<2 h）七氟醚引起的神经元凋亡无明显神经行为学意义。
목적：연구단시간흡입칠불미대발육중대서해마신경원조망적영향。방법78지1개월령 SD대서안수궤수자표법수궤분위3조，공기대조조（Sa，26지），재기대조조（Sc，26지），3％칠불미흡입2 h 조（S2，26지），건립칠불미흡입모형，흡입결속후12 h 용광장실험화 T 미궁진행신경행위학측시，동시단두취해마조직，소목소-이홍염색관찰해마신경원형태학개변，용류식세포의검측조망，격광공취초현미경측신경원세포내유리개리자농도。결과 S2조해마조망신경원수목이급신경원내유리개리자상대형광상소치균고우대조조，차이유통계학의의（P<0.05）；단신경행위학검측무명현차별。결론칠불미가유도유령대서해마신경원조망，가능통과승고세포내개리자농도이촉발조망。단단차、단시간흡입（<2 h）칠불미인기적신경원조망무명현신경행위학의의。
Objective To study the effects of short-term inhalation of sevoflurane on the hippocampal neuronal apoptosis of rats during puberty. Methods Seventy-eight Sprague-Dawley (SD) rats aged 1 month were randomly as-signed to 3 groups: air control group (Sa, n=26), carrier gas control group (Sc, n=26), 2 h inhalation of 3% sevoflurane group (S2, n=26). The inhalation of sevoflurane model was established. At 12 h after inhalation, all groups underwent neuroethology asessment using open-field and T-maze test. This was followed by decapitation for extraction of the hip-pocampus. The changes of cell morphology in hippocampal neuron were observed via HE staining. Apoptotic cells were measured by flow cytometry, and laser scanning, and confocal microscope was used to measure the concentration of intracellular calcium in the neuron. Results The number of apoptotic neuron, intracellular free calcium concentra-tion and fluorescence intensity in group S2 were significantly increased compared with control groups (all P<0.05). Conclusion Sevoflurane elicits hippocampal neuron apoptosis in rat infants, possibly via increasing the concentration of intracellular calcium. However, neuron apoptosis caused by a single short-time inhalation of sevoflurane (<2 h) did not markedly affect the neuroethology.