CAJ | 학술논문

目的：检测 Octamer 4（Oct-4）基因在非小细胞肺癌（NSCLC）中的表达，探讨 Oct-4在 NSCLC 发生、发展中的作用。方法采用流式细胞仪分选出肺癌细胞系 A549中 Oct-4＋及 Oct-4-的细胞群，用免疫荧光法检测分选后的细胞亚群中 CD133的表达情况；采用体外侵袭实验检测 Oct-4＋细胞群和 Oct-4-细胞群的侵袭能力。结果①以标记 Oct-4通过流式细胞仪分离后，成功获得肺癌细胞株 A549中 Oct-4＋细胞群和 Oct-4-细胞群。且Oct-4＋细胞群中 CD133的表达明显高于 Oct-4-细胞群，差异有统计学意义（P<0.05）。②Oct-4＋细胞群的侵袭细胞数显著高于 Oct-4-细胞群，差异有统计学意义（P<0.05）。③Oct-4在 NSCLC 中的表达率为83％（50/60），且 Oct-4＋细胞群中 CD133的表达明显高于 Oct-4-细胞群，两者比较差异有统计学意义（P<0.05）；且 Oct-4的表达与NSCLC 组织的分化程度、肿瘤的临床分期及是否已经发生淋巴结转移有关，差异有统计学意义（P<0.05）。结论干细胞标志物 Oct-4在 NSCLC 干细胞亚群中呈高表达，可能与肺癌的发生、发展及转移密切相关，为肺癌治疗提供新的靶点。
목적：검측 Octamer 4（Oct-4）기인재비소세포폐암（NSCLC）중적표체，탐토 Oct-4재 NSCLC 발생、발전중적작용。방법채용류식세포의분선출폐암세포계 A549중 Oct-4＋급 Oct-4-적세포군，용면역형광법검측분선후적세포아군중 CD133적표체정황；채용체외침습실험검측 Oct-4＋세포군화 Oct-4-세포군적침습능력。결과①이표기 Oct-4통과류식세포의분리후，성공획득폐암세포주 A549중 Oct-4＋세포군화 Oct-4-세포군。차Oct-4＋세포군중 CD133적표체명현고우 Oct-4-세포군，차이유통계학의의（P<0.05）。②Oct-4＋세포군적침습세포수현저고우 Oct-4-세포군，차이유통계학의의（P<0.05）。③Oct-4재 NSCLC 중적표체솔위83％（50/60），차 Oct-4＋세포군중 CD133적표체명현고우 Oct-4-세포군，량자비교차이유통계학의의（P<0.05）；차 Oct-4적표체여NSCLC 조직적분화정도、종류적림상분기급시부이경발생림파결전이유관，차이유통계학의의（P<0.05）。결론간세포표지물 Oct-4재 NSCLC 간세포아군중정고표체，가능여폐암적발생、발전급전이밀절상관，위폐암치료제공신적파점。
Objective To detect the expression of Octamer 4 (Oct-4) in non-small cell lung cancer (NSCLC), and to explore its role in the pathogensis and development of NSCLC. Methods Oct-4+ and Oct-4- cells in lung can-cer cell line A549 were selected by using flow cytometry, and the expression of sorted cells CD133 were detected by immumofluorescence assay. Invasion assay in vitro was adopted to detect the invasive capacity of Oct-4+ cell subsets and Oct-4- cell subsets. Results After separation of Oct-4 labeled with flow cytometry, Oct-4+ cell subsets and Oct-4-cell subsets in lung cancer cell line A549 were obtained. The expression of CD133 in Oct-4+ cell subsets was signifi-cantly higher than that of Oct-4- cell subsets (P<0.05). The number of invasion cells of Oct-4+ cell subsets was signifi-cantly higher than that of Oct-4- cell subsets (P<0.05). The expression rate of Oct-4 in NSCLC was 83% (50/60). The expression of CD133 in Oct-4+ cell subsets was significantly higher than that in Oct-4- cell subsets (P<0.05). The ex-pression of Oct-4 was related with the differentiation of NSCLC tissue, clinical staging of tumor and the occurrence of lymph node metastasis (P<0.05). Conclusion The stem cell markers Oct-4 showed high expression in NSCLC stem cell subpopulations, which may be closely associated with the pathogenesis, development and metastasis of lung can-cer, thus offering a novel target for the treatment of lung cancer.