中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
10期
1788-1789
,共2页
高效液相色谱法%氯霉素%阴道片%含量测定
高效液相色譜法%氯黴素%陰道片%含量測定
고효액상색보법%록매소%음도편%함량측정
HPLC%Chloramphenicol%Vaginaltablets%Determination
目的::建立测定氯霉素阴道片中氯霉素含量的 HPLC法。方法:色谱柱为 Agilent ZORBAX SB-C18柱(150 mm ×4.6 mm,5μm),流动相为含0.1%庚烷磺酸钠的溶液-甲醇(68∶32),流速1 ml·min-1,检测波长277 nm,柱温35℃,进样量10μl。结果:氯霉素浓度在25.6~512.0μg·ml-1范围内与峰面积线性关系良好,r=0.9999,平均回收率为99.4%,RSD为0.8%(n=9)。结论:该法简便、准确、重复性好,适用于测定氯霉素阴道片中氯霉素的含量。
目的::建立測定氯黴素陰道片中氯黴素含量的 HPLC法。方法:色譜柱為 Agilent ZORBAX SB-C18柱(150 mm ×4.6 mm,5μm),流動相為含0.1%庚烷磺痠鈉的溶液-甲醇(68∶32),流速1 ml·min-1,檢測波長277 nm,柱溫35℃,進樣量10μl。結果:氯黴素濃度在25.6~512.0μg·ml-1範圍內與峰麵積線性關繫良好,r=0.9999,平均迴收率為99.4%,RSD為0.8%(n=9)。結論:該法簡便、準確、重複性好,適用于測定氯黴素陰道片中氯黴素的含量。
목적::건립측정록매소음도편중록매소함량적 HPLC법。방법:색보주위 Agilent ZORBAX SB-C18주(150 mm ×4.6 mm,5μm),류동상위함0.1%경완광산납적용액-갑순(68∶32),류속1 ml·min-1,검측파장277 nm,주온35℃,진양량10μl。결과:록매소농도재25.6~512.0μg·ml-1범위내여봉면적선성관계량호,r=0.9999,평균회수솔위99.4%,RSD위0.8%(n=9)。결론:해법간편、준학、중복성호,괄용우측정록매소음도편중록매소적함량。
Objective:To establish an HPLC method for the determination of chloramphenicol in chloramphenicol vaginal tablets. Methods:An Agilent ZORBAX SB-C18 column (150 mm × 4. 6 mm,5 μm)was used. The mobile phase was composed of 0. 1% sodi-um 1-heptanesulfonate solution-methanol (68∶32),the flow rate was 1. 0 ml·min-1, the UV detection wavelength was 277 nm,the column temperature was 35℃,and the injection volume was 10 μl. Results:The linear range of chloramphenicol was 25. 6-512. 0 μg ·ml-1(r=0. 999 9). The mean recovery was 99. 4%, and RSD was 0. 8%(n=9). Conclusion:The method is simple,accurate and reproducible,and can be used in the determination of chloramphenicol vaginal tablets.