中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
10期
1786-1787
,共2页
大叶金花草%牡荆素%高效液相色谱法%含量测定
大葉金花草%牡荊素%高效液相色譜法%含量測定
대협금화초%모형소%고효액상색보법%함량측정
Stenoloma chusana( L. ) Citing%Viterxin%HPLC%Determination
目的：：建立高效液相色谱法测定大叶金花草中牡荆素含量。方法：采用Waters Symmetry C18(250 mm ×4.6 mm,5μm)色谱柱,流动相为乙腈-0.1%冰乙酸溶液(27∶73),流速为1.0 ml·min-1,检测波长为339 nm,柱温为30℃。结果：牡荆素浓度在10.03~90.27μg·ml-1范围内与峰面积的线性关系良好(r=0.9999),平均回收率为100.48%,RSD为0.98%。结论：本分析方法准确、灵敏、重现性好,可用于大叶金花草中牡荆素的含量测定。
目的：：建立高效液相色譜法測定大葉金花草中牡荊素含量。方法：採用Waters Symmetry C18(250 mm ×4.6 mm,5μm)色譜柱,流動相為乙腈-0.1%冰乙痠溶液(27∶73),流速為1.0 ml·min-1,檢測波長為339 nm,柱溫為30℃。結果：牡荊素濃度在10.03~90.27μg·ml-1範圍內與峰麵積的線性關繫良好(r=0.9999),平均迴收率為100.48%,RSD為0.98%。結論：本分析方法準確、靈敏、重現性好,可用于大葉金花草中牡荊素的含量測定。
목적：：건립고효액상색보법측정대협금화초중모형소함량。방법：채용Waters Symmetry C18(250 mm ×4.6 mm,5μm)색보주,류동상위을정-0.1%빙을산용액(27∶73),류속위1.0 ml·min-1,검측파장위339 nm,주온위30℃。결과：모형소농도재10.03~90.27μg·ml-1범위내여봉면적적선성관계량호(r=0.9999),평균회수솔위100.48%,RSD위0.98%。결론：본분석방법준학、령민、중현성호,가용우대협금화초중모형소적함량측정。
Objective:To establish an HPLC method for the content determination of viterxin in Stenoloma chusana( L. ) Citing. Methods:A Waters Symmetry C18 (250 mm × 4. 6 mm, 5 μm) column was used, and the mobile phase was acetonitrile-0. 1% glacial acetic acid solution (27∶73) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was at 339nm, and the column temperature was 30℃. Results:The linear relationship of viterxin was good within the range from 10. 03 to 90. 27μg·ml-1 . The average recovery was 100. 48%(RSD=0. 98%). Conclusion:The established method is accurate and sensitive with good reproducibility, which can be applied in the determination of viterxin of Stenoloma chusana( L. ) Citing.
