中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
10期
1782-1783
,共2页
何崇慧%高磊%贾燕花%王晓青%刘皈阳
何崇慧%高磊%賈燕花%王曉青%劉皈暘
하숭혜%고뢰%가연화%왕효청%류귀양
麝香草酚%麝香草酚醇溶液%高效液相色谱法%含量测定
麝香草酚%麝香草酚醇溶液%高效液相色譜法%含量測定
사향초분%사향초분순용액%고효액상색보법%함량측정
Thymol%Thymolalcoholicsolution%HPLC%Contentdetermination
目的：：建立 HPLC 法测定麝香草酚醇溶液中麝香草酚的含量。方法：采用 Agilent Eclipse XDB-C18(250 mm ×4.6 mm,5μm)色谱柱,甲醇-水(65∶35)为流动相,流速为1.0 ml·min-1,检测波长为275 nm,进样量为10μl,柱温为25℃。结果：麝香草酚在60~160μg·ml-1范围内与其峰面积线性关系良好(r=0.9995),平均回收率为101.59%(n=9),RSD值为1.39%。结论：本方法简便快捷、准确度高、专属性强、灵敏度高,可用于麝香草酚醇溶液中麝香草酚的含量测定。
目的：：建立 HPLC 法測定麝香草酚醇溶液中麝香草酚的含量。方法：採用 Agilent Eclipse XDB-C18(250 mm ×4.6 mm,5μm)色譜柱,甲醇-水(65∶35)為流動相,流速為1.0 ml·min-1,檢測波長為275 nm,進樣量為10μl,柱溫為25℃。結果：麝香草酚在60~160μg·ml-1範圍內與其峰麵積線性關繫良好(r=0.9995),平均迴收率為101.59%(n=9),RSD值為1.39%。結論：本方法簡便快捷、準確度高、專屬性彊、靈敏度高,可用于麝香草酚醇溶液中麝香草酚的含量測定。
목적：：건립 HPLC 법측정사향초분순용액중사향초분적함량。방법：채용 Agilent Eclipse XDB-C18(250 mm ×4.6 mm,5μm)색보주,갑순-수(65∶35)위류동상,류속위1.0 ml·min-1,검측파장위275 nm,진양량위10μl,주온위25℃。결과：사향초분재60~160μg·ml-1범위내여기봉면적선성관계량호(r=0.9995),평균회수솔위101.59%(n=9),RSD치위1.39%。결론：본방법간편쾌첩、준학도고、전속성강、령민도고,가용우사향초분순용액중사향초분적함량측정。
Objective:To establish an HPLC method for the determination of thymol in thymol alcoholic solutions. Methods: An Agilent Eclipse XDB-C18(250 mm ×4.6 mm,5μm) column was used with the mobile phase of methanol-water (65∶35), the flow rate was 1. 0 ml·min-1 . the detection wavelength was 275nm, the injection volume was 10μl, and the column tenperature was 25℃. Re-sults:A good linear correlation of thymol was observed within the range of 60-160 μg·ml-1(r=0. 999 5). The average recovery was 101. 59% with RSD of 1. 39%(n=9). Conclusion:The method is quick, simple and accurate, which can be used in the determina-tion of thymol alcoholic solutions with good selectivity and sensitivity.
