中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
10期
1778-1779
,共2页
苯扎氯铵%高效液相色谱法%复方门冬维甘滴眼液%含量测定
苯扎氯銨%高效液相色譜法%複方門鼕維甘滴眼液%含量測定
분찰록안%고효액상색보법%복방문동유감적안액%함량측정
Benzalkonium chloride%HPLC%Compound aspartate,vitamin B6 and dipotassium glycyrrhetate eye drops%Determination
目的：：建立测定复方门冬维甘滴眼液中苯扎氯铵含量的方法。方法：采用Agilent Zorbax C18色谱柱(150 mm ×4.6 mm,5μm),以乙腈-5 mmol·L-1醋酸铵溶液(含1%三乙胺,用冰醋酸调节pH至5.0±0.5)(65∶35)为流动相,流速为1.0 ml·min-1,检测波长为214 nm。结果：苯扎氯铵在50.00~150.00μg·ml-1范围内与峰面积线性关系良好,r=0.9980；平均回收率为99.1%,RSD为0.6%。结论：该方法简便,迅速,灵敏度高,具有良好的重复性及回收率,可用于复方门冬维甘滴眼液中苯扎氯铵的测定。
目的：：建立測定複方門鼕維甘滴眼液中苯扎氯銨含量的方法。方法：採用Agilent Zorbax C18色譜柱(150 mm ×4.6 mm,5μm),以乙腈-5 mmol·L-1醋痠銨溶液(含1%三乙胺,用冰醋痠調節pH至5.0±0.5)(65∶35)為流動相,流速為1.0 ml·min-1,檢測波長為214 nm。結果：苯扎氯銨在50.00~150.00μg·ml-1範圍內與峰麵積線性關繫良好,r=0.9980；平均迴收率為99.1%,RSD為0.6%。結論：該方法簡便,迅速,靈敏度高,具有良好的重複性及迴收率,可用于複方門鼕維甘滴眼液中苯扎氯銨的測定。
목적：：건립측정복방문동유감적안액중분찰록안함량적방법。방법：채용Agilent Zorbax C18색보주(150 mm ×4.6 mm,5μm),이을정-5 mmol·L-1작산안용액(함1%삼을알,용빙작산조절pH지5.0±0.5)(65∶35)위류동상,류속위1.0 ml·min-1,검측파장위214 nm。결과：분찰록안재50.00~150.00μg·ml-1범위내여봉면적선성관계량호,r=0.9980；평균회수솔위99.1%,RSD위0.6%。결론：해방법간편,신속,령민도고,구유량호적중복성급회수솔,가용우복방문동유감적안액중분찰록안적측정。
Objective:To develop a method to determine benzalkonium chloride in compound aspartate, vitamin B6 and dipotassi-umglycyrrhetateeyedrops.Methods:TheseparationwasperformedonanAgilentZorbaxC18(150mm×4.6mm,5μm)column. The mobile phase consisted of acetonitrile-5 mmol·L-1 ammonium acetate ( in which 1% triethylamine was added, then the pH value was adjusted to 5. 0 ± 0. 5 by acetic acid) 65∶35(v/v). The flow rate was 1. 0 ml·min-1 and the detection wavelength was 214nm. Re-sults:Benzalkonium chloride had a good linearity within the range of 50. 00-150. 00μg·ml-1(r=0. 998 0), and the average recovery was 99. 1%(RSD=0. 6%). Conclusion:The method is simple, rapid and sensitive with good repeatability and recovery. It can be used to determine benzalkoinum chloride in compound aspartate, vitamin B6 and dipotassium glycyrrhetate eye drops.
