中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
10期
1772-1773,1774
,共3页
刘亚萍%张燕%徐红%周星彤%杨钊
劉亞萍%張燕%徐紅%週星彤%楊釗
류아평%장연%서홍%주성동%양쇠
3,5-二硝基水杨酸%还原糖%羟乙基淀粉%紫外光度法
3,5-二硝基水楊痠%還原糖%羥乙基澱粉%紫外光度法
3,5-이초기수양산%환원당%간을기정분%자외광도법
DNS%Reducingsugar%Hydroxyethylstarch%UV
目的::研究3,5-二硝基水杨酸(DNS)法测定羟乙基淀粉还原端的条件。方法:以葡萄糖做标准曲线来测定羟乙基淀粉还原端的含量,研究DNS试剂的用量、加热温度、加热时间及反应后放置时间对测定结果的影响。结果: DNS法测定羟乙基淀粉的最佳反应条件是:DNS试剂用量0.8 ml,反应温度85℃,反应时间5 min,显色定容后于540 nm的波长处测定吸光度。结论:本方法操作简单、准确,重复性好,可用于羟乙基淀粉还原端的测定。
目的::研究3,5-二硝基水楊痠(DNS)法測定羥乙基澱粉還原耑的條件。方法:以葡萄糖做標準麯線來測定羥乙基澱粉還原耑的含量,研究DNS試劑的用量、加熱溫度、加熱時間及反應後放置時間對測定結果的影響。結果: DNS法測定羥乙基澱粉的最佳反應條件是:DNS試劑用量0.8 ml,反應溫度85℃,反應時間5 min,顯色定容後于540 nm的波長處測定吸光度。結論:本方法操作簡單、準確,重複性好,可用于羥乙基澱粉還原耑的測定。
목적::연구3,5-이초기수양산(DNS)법측정간을기정분환원단적조건。방법:이포도당주표준곡선래측정간을기정분환원단적함량,연구DNS시제적용량、가열온도、가열시간급반응후방치시간대측정결과적영향。결과: DNS법측정간을기정분적최가반응조건시:DNS시제용량0.8 ml,반응온도85℃,반응시간5 min,현색정용후우540 nm적파장처측정흡광도。결론:본방법조작간단、준학,중복성호,가용우간을기정분환원단적측정。
Objective:To explore the determination conditions for reducing end of hydroxyethyl starch by DNS spectrophotometry. Methods:The reducing end of hydroxyethyl starch was determined using the standard curve of glucose solutions. The effects of DNS reagent with different volume, heating temperature, heating time and standing time after reaction on the determination were investiga-ted. Results:The optimal determination conditions were as follows:the DNS volume was 0. 8 ml, the reaction temperature was 85℃, the reaction time was 5 minutes, and the colored solution was determined at the wavelength of 540 nm. Conclusion: The method is simple and accurate with good reproducibility, which can be used to determine reducing end of hydroxyethyl starch.