CAJ | 학술논문

从奶牛组织中克隆Tenomodulin（TNMD)基因的cDNA序列，采用双酶切后连接表达载体pGEX-4T-1，转入大肠杆菌BL21中进行诱导表达。结果表明，TNMD基因的序列全长为957 bp，通过双酶切构建的表达载体pGEX-TNMD在BL21大肠杆菌中成功表达了分子量为59.68 kDa的融合蛋白。
종내우조직중극륭Tenomodulin（TNMD)기인적cDNA서렬，채용쌍매절후련접표체재체pGEX-4T-1，전입대장간균BL21중진행유도표체。결과표명，TNMD기인적서렬전장위957 bp，통과쌍매절구건적표체재체pGEX-TNMD재BL21대장간균중성공표체료분자량위59.68 kDa적융합단백。
The Tenomodulin (TNMD﹚ cDNA of bovine was amplified by RT-CR. Then the gene fragment was digested with enzyme and cloned into expression vector pGEX-4T-1. The recombinant vector was transformed into E. coli BL21. The results showed that the full-length of the TNMD gene sequence was 957 bp. The reconstruction plasmid pGEX-TNMD was constructed successfully and a 59.68 kDa fusion protein was expressed in E. coli BL21 induced by IPTG.