湖北农业科学
湖北農業科學
호북농업과학
2014年
7期
1654-1656
,共3页
高效液相色谱%瑞香素%芫花素%唐古特瑞香(Dap hne tangutic a)
高效液相色譜%瑞香素%芫花素%唐古特瑞香(Dap hne tangutic a)
고효액상색보%서향소%원화소%당고특서향(Dap hne tangutic a)
HPLC%daphnetin%genkwanin%Daphne tangutica
建立同时测定唐古特瑞香（Dap hne tangutic a)中瑞香素和芫花素的含量的HPLC方法。采用Agi-lent ZORBAX（4.6 mm×250 mm，5μm)色谱柱；检测波长为360 nm；柱温为30℃。结果表明，瑞香素在0.115~0.920μg内线性关系良好（R2=0.9999)，其平均回收率为99.19%，RSD=1.2%（n=9)。芫花素在0.011~0.088μg内线性关系良好（R2=0.9999)，其平均回收率为100.25%，RSD=1.5%（n=9)。该方法样品处理简单、准确度高、分离效果好，适用于该药的成分测定。
建立同時測定唐古特瑞香（Dap hne tangutic a)中瑞香素和芫花素的含量的HPLC方法。採用Agi-lent ZORBAX（4.6 mm×250 mm，5μm)色譜柱；檢測波長為360 nm；柱溫為30℃。結果錶明，瑞香素在0.115~0.920μg內線性關繫良好（R2=0.9999)，其平均迴收率為99.19%，RSD=1.2%（n=9)。芫花素在0.011~0.088μg內線性關繫良好（R2=0.9999)，其平均迴收率為100.25%，RSD=1.5%（n=9)。該方法樣品處理簡單、準確度高、分離效果好，適用于該藥的成分測定。
건립동시측정당고특서향（Dap hne tangutic a)중서향소화원화소적함량적HPLC방법。채용Agi-lent ZORBAX（4.6 mm×250 mm，5μm)색보주；검측파장위360 nm；주온위30℃。결과표명，서향소재0.115~0.920μg내선성관계량호（R2=0.9999)，기평균회수솔위99.19%，RSD=1.2%（n=9)。원화소재0.011~0.088μg내선성관계량호（R2=0.9999)，기평균회수솔위100.25%，RSD=1.5%（n=9)。해방법양품처리간단、준학도고、분리효과호，괄용우해약적성분측정。
The high performance liquid chromatography (HPLC﹚ was used to simultaneously determine daphnetin and genkwanin in Daphne tangutica. The samples were separated on Agilent ZORBAX (4.6 mm×250 mm,5μm﹚ column. Detection wavelength was 360 nm and column temperature was 30℃. The results showed that the linear range of daphnetin was good in 0.115~0.920μg (R2=0.999 9﹚ with the average recovery of 99.19% and RSD of 1.2%(n=9). The linear range of genkwanin was good in 0.011~0.088μg (R2=0.999 9﹚ with the average recovery of 100.25% and RSD of 1.5%(n=9﹚. This method was simple, accurate,and suitable for determining components and quantities of daphnetin and genkwanin in Daphne tangutica.
