CAJ | 학술논문

利用3因素5水平的中心组合试验设计优化了玉米秸秆的纤维素酶水解条件，以提高纤维素氢气的产量。结果表明，酶解温度、pH和酶用量对氢气产量具有显著影响。在酶解温度51.8℃，pH 4.84、酶用量9.00 FPU/g TS 条件下，氢气产量达到208.1 mL/g TVS。通过分析玉米秸秆在降解过程中的成分变化，发现玉米秸秆经水解后纤维素和半纤维素被降解，产氢菌主要利用其产生的可溶性糖发酵产氢，且混合产氢菌也具有直接降解纤维素发酵产氢的能力。
이용3인소5수평적중심조합시험설계우화료옥미갈간적섬유소매수해조건，이제고섬유소경기적산량。결과표명，매해온도、pH화매용량대경기산량구유현저영향。재매해온도51.8℃，pH 4.84、매용량9.00 FPU/g TS 조건하，경기산량체도208.1 mL/g TVS。통과분석옥미갈간재강해과정중적성분변화，발현옥미갈간경수해후섬유소화반섬유소피강해，산경균주요이용기산생적가용성당발효산경，차혼합산경균야구유직접강해섬유소발효산경적능력。
The cellulosic hydrogen production from cellulase hydrolysis cornstalk was studied using response surface methodol-ogy (RSM﹚. The parameters of process for enzymatic hydrolysis of cornstalk were optimized by a three factor-five level central composite design. The results showed that the optimal parameters of enzymatic hydrolysis of substrate were temperature 51.8℃, pH 4.84 and 9.00 FPU/g TS of enzyme loading. The maximum hydrogen yield from cornstalk by anaerobic mixed mi-croflora was 208.1 ml/g TVS under the optimal condition. The hydrogen was attributed to the bioconversion of the generated soluble sugars from the acid-enzymatic hydrolysis of corn stalk. The hydrogen-producing microflora has the ability to directly degrade cellulose to produce hydrogen.