CAJ | 학술논문

为了解奶牛S100A 12基因序列的特征及其在乳腺和生殖系统中的表达情况，采用RT-PCR技术从奶牛外周血液中扩增S100A 12基因，重组到pMD19-T Simple载体中，进行了测序和序列分析，并应用RT-PCR技术检测了奶牛乳腺和生殖系统（卵巢、输卵管、子宫颈、子宫体、阴道等组织)中S100A12 mR-NA的表达分布。结果显示，克隆的S100A 12基因包含一个279 bp的完整开放阅读框（ORF)，与GenBank中的奶牛S100A12基因（NM 174651)编码区序列100%相同，该ORF编码的92个氨基酸含有一个EF手型结构（位于49-84氨基酸残基)；另外，还预测到3个蛋白激酶C磷酸化位点，分别位于19-21、44-46和70-73氨基酸残基。S100A 12基因在奶牛乳腺和生殖系统上皮组织中均有表达。推测S100A 12基因在奶牛乳腺和生殖系统中具有一定作用。
위료해내우S100A 12기인서렬적특정급기재유선화생식계통중적표체정황，채용RT-PCR기술종내우외주혈액중확증S100A 12기인，중조도pMD19-T Simple재체중，진행료측서화서렬분석，병응용RT-PCR기술검측료내우유선화생식계통（란소、수란관、자궁경、자궁체、음도등조직)중S100A12 mR-NA적표체분포。결과현시，극륭적S100A 12기인포함일개279 bp적완정개방열독광（ORF)，여GenBank중적내우S100A12기인（NM 174651)편마구서렬100%상동，해ORF편마적92개안기산함유일개EF수형결구（위우49-84안기산잔기)；령외，환예측도3개단백격매C린산화위점，분별위우19-21、44-46화70-73안기산잔기。S100A 12기인재내우유선화생식계통상피조직중균유표체。추측S100A 12기인재내우유선화생식계통중구유일정작용。
To study the characteristics of dairy cow S100A 12 gene and its expression in lacteal gland and genital system, the S100A 12 gene was amplified by RT-PCR from peripheral blood of dairy cow and sequenced and analyzed after recombina-tion in pMD19-T Simple vector. The expression distributions of S100A 12 mRNA in dairy cow’s lacteal gland and genital system were detected by RT-PCR. The results showed that the cloned S100A 12 gene had a 270bp ORF, 100% identical with S100A 12 gene in GenBank. In ORF, there was a EF hand formation consisted of 92 amino acids (49-84 amino acids residue﹚. Three phosphorylation sites of protein kinase C were found in 19-21, 44-46, 70-73 amino acid residues. S100A 12 gene was expressed in all epithelial tissue of dairy cow lacteal gland and genital system. It might play an important role in dairy cow lacteal gland and genital system.