CAJ | 학술논문

试验采用透析法提取了猪脾转移因子（TF)，并对其理化特性、生物安全性以及其对非特异性和特异性免疫的影响等方面进行了初步研究。结果表明，利用透析法制备的猪脾TF能够显著（P<0.05)增强小鼠腹腔巨噬细胞的吞噬能力；对于E玫瑰花环的形成数和形成率具有极显著（P<0.01)的促进作用；将猪脾TF与猪瘟疫苗同时使用，能够显著（P<0.05)提高机体体液免疫的抗体滴度。此外，制备的猪脾TF安全无毒、无抗原性，不会使异种动物体内产生相应抗体。表明猪脾TF对于机体的特异性和非特异性免疫均有促进作用，是一种安全的生物免疫调节剂。
시험채용투석법제취료저비전이인자（TF)，병대기이화특성、생물안전성이급기대비특이성화특이성면역적영향등방면진행료초보연구。결과표명，이용투석법제비적저비TF능구현저（P<0.05)증강소서복강거서세포적탄서능력；대우E매괴화배적형성수화형성솔구유겁현저（P<0.01)적촉진작용；장저비TF여저온역묘동시사용，능구현저（P<0.05)제고궤체체액면역적항체적도。차외，제비적저비TF안전무독、무항원성，불회사이충동물체내산생상응항체。표명저비TF대우궤체적특이성화비특이성면역균유촉진작용，시일충안전적생물면역조절제。
The transfer factors (TFs﹚ of porcine spleen were extracted by dialysis method and studied on the physical and chemical properties, biological security, and its effects on non-specific and specific immune. The results showed that TFs significantly enhanced the mouse’s peritoneal macrophage phagocytosis (P<0.05﹚,and extremely increased the E rosette formation number and rate (P<0.01﹚. Used with classical swine fever vaccine, the antibody titer of humoral immunity prominently increased (P<0.05﹚. In addition,the extracted TFs was safe and non-antigenic. The disparate animals had no antibodies produced. It is indicated that TFs of porcine spleen could promote the body's non-specific and specific immune, and was a safe biological immunomodulators. With the further research of TFs,the application of it in controlling animal disease will be more extensive.