CAJ | 학술논문

为了分析蛋白质变性剂和金属离子对从中华皮蝇（Hypoderma sinense)幼虫中纯化的单体乳酸脱氢酶（LDH)活力的影响，分别用3种蛋白质变性剂[尿素、盐酸胍、十二烷基硫酸钠（SDS)]以及8种二价金属离子在体外处理纯化的中华皮蝇LDH，再测定其酶活力。结果表明，中华皮蝇LDH对蛋白质变性剂尿素有一定的耐受性，对SDS敏感；在1~20 mmol/L浓度范围内，大多数二价金属离子对LDH活力有不同程度的抑制作用，但3 mmol/L的Co2+对LDH活力有很强的促进作用，这不同于其他来源的LDH。该研究结果提示中华皮蝇LDH具有较高的抗变性能力，对高浓度Co2+的需求可能是其重要特征之一。
위료분석단백질변성제화금속리자대종중화피승（Hypoderma sinense)유충중순화적단체유산탈경매（LDH)활력적영향，분별용3충단백질변성제[뇨소、염산고、십이완기류산납（SDS)]이급8충이개금속리자재체외처리순화적중화피승LDH，재측정기매활력。결과표명，중화피승LDH대단백질변성제뇨소유일정적내수성，대SDS민감；재1~20 mmol/L농도범위내，대다수이개금속리자대LDH활력유불동정도적억제작용，단3 mmol/L적Co2+대LDH활력유흔강적촉진작용，저불동우기타래원적LDH。해연구결과제시중화피승LDH구유교고적항변성능력，대고농도Co2+적수구가능시기중요특정지일。
To study effects of three protein denaturants and eight metal ions on the activities of monomeric lactate dehydro-genase (LDH﹚ purified from Hypoderma sinense larva, the purified LDH was incubated in vitro with urea, sodium dodecyl sulfate (SDS﹚, guanidine hydrochloride and eight divalent metal ions, and then subjected to activity analysis. The results showed that Hypoderma sinense LDH had relative resistance to urea, but was sensitive to sodium dodecyl sulfate (SDS﹚. Most metal ions had varied degrees of inhibition to LDH activity at concentrations from 1 to 20 mmol/L, while Co2+ in-creased LDH activity significantly at 3 mmol/L concentration, quite different from the LDH of other treats. It is indicated that Hypoderma sinense LDH had strong ability against denaturalization and its requirement for high concentration of Co2+might be its one important property.