CAJ | 학술논문

采用纯培养的方法，从美国内达华州温泉和中国福建永泰温泉分离得到27株嗜热细菌，从中筛选得到产纤维素酶的嗜热细菌并进行了16S rDNA鉴定。结果表明，LY7和LY8是产纤维素酶的嗜热细菌，菌株 LY7与脂环酸芽孢杆菌属（Alicyclobacillus )的同源性达到99%，菌株 LY8与土芽孢杆菌属（Geobacillus)的同源性达到99%，生长温度范围在40~70°C之间，最适温度65°C。酶学性质分析表明， LY8的内切酶酶活高达145 IU/mL。
채용순배양적방법，종미국내체화주온천화중국복건영태온천분리득도27주기열세균，종중사선득도산섬유소매적기열세균병진행료16S rDNA감정。결과표명，LY7화LY8시산섬유소매적기열세균，균주 LY7여지배산아포간균속（Alicyclobacillus )적동원성체도99%，균주 LY8여토아포간균속（Geobacillus)적동원성체도99%，생장온도범위재40~70°C지간，최괄온도65°C。매학성질분석표명， LY8적내절매매활고체145 IU/mL。
Culture-based approach was used to isolate thermophiles from hot spring. In total, 27 thermophilic bacterial strains were isolated from hot springs in Nevada of USA and Yongtai hot spring in Fujian province of China. Superior cellulose and hemicellulose decomposing strains were screened and identified by 16S rDNA. The results showed that LY7 and LY8 degrad-ing cellulose were indentified as Alicyclobacillus sp. LY7 and Geobacillus sp. LY8. Geobacillus sp. LY8 could be cultured at temperature ranging from 40℃ to 70℃, with the optimal temperature of 65℃. The β-glucosidase activity of LY8 was 145 IU/mL.