湖北农业科学
湖北農業科學
호북농업과학
2014年
7期
1506-1508,1512
,共4页
玉米(Zea mays L.)地方品种%核心种质%遗传多样性%武陵山区
玉米(Zea mays L.)地方品種%覈心種質%遺傳多樣性%武陵山區
옥미(Zea mays L.)지방품충%핵심충질%유전다양성%무릉산구
maize(Zea mays L.) landraces%the core collection%genetic diversity%Wuling mountain area
田间试验结合微卫星（ SSR )标记技术，从表型和 DNA 分子水平分析评价了武陵山区玉米（Zea mays L.)地方品种核心种质的遗传多样性。结果表明，核心种质在14个农艺、经济性状上存在极显著差异；基于SSR标记的UPGMA聚类能有效区分玉米地方品种核心种质，50对SSR引物在核心种质中共检测到275个多态性位点，各引物检测到的等位位点数3~15个，平均为6.0个；Shannon多态信息量为0.29~0.83，平均为0.75。核心种质代表性评价结果表明核心种质较好地保持了原种质库的遗传变异。
田間試驗結閤微衛星（ SSR )標記技術，從錶型和 DNA 分子水平分析評價瞭武陵山區玉米（Zea mays L.)地方品種覈心種質的遺傳多樣性。結果錶明，覈心種質在14箇農藝、經濟性狀上存在極顯著差異；基于SSR標記的UPGMA聚類能有效區分玉米地方品種覈心種質，50對SSR引物在覈心種質中共檢測到275箇多態性位點，各引物檢測到的等位位點數3~15箇，平均為6.0箇；Shannon多態信息量為0.29~0.83，平均為0.75。覈心種質代錶性評價結果錶明覈心種質較好地保持瞭原種質庫的遺傳變異。
전간시험결합미위성（ SSR )표기기술，종표형화 DNA 분자수평분석평개료무릉산구옥미（Zea mays L.)지방품충핵심충질적유전다양성。결과표명，핵심충질재14개농예、경제성상상존재겁현저차이；기우SSR표기적UPGMA취류능유효구분옥미지방품충핵심충질，50대SSR인물재핵심충질중공검측도275개다태성위점，각인물검측도적등위위점수3~15개，평균위6.0개；Shannon다태신식량위0.29~0.83，평균위0.75。핵심충질대표성평개결과표명핵심충질교호지보지료원충질고적유전변이。
Using field experiment and microsatellite (SSR﹚ analysis, the genetic diversity of the core germplasms of maize landraces from Wuling mountain area was evaluated at the morphological level and DNA molecular level. The results showed that significant differences were found in 14 agronomic and economic traits of the core collection. On the basis of SSR data, the core collection can be distinguished by clustering analysis. 275 polymorphic alleles were detected. At each locus, the mean number of alleles was 6.0 ranging from 3 to 15. The Shannon polymorphism information content was varied from 0.29 to 0.83, with an average of 0.75. It is indicated the genetic diversity of the core collection represented that of the initial collection.
