中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
35期
23-25
,共3页
癌,肝细胞%T淋巴细胞%消融治疗
癌,肝細胞%T淋巴細胞%消融治療
암,간세포%T림파세포%소융치료
Carcinoma,hepatocellular%T-lymphocytes%Thermal ablation treatment
目的 探讨肝癌患者热消融治疗对调节性T淋巴细胞(Tregs)的影响及其提高机体抗肿瘤免疫作用的相关机制.方法 选择2009年12月至2010年12月行微波消融治疗的肝癌患者36例(观察组),并选择同期健康体检者20例作为对照组.采用流式细胞仪检测Tregs;采用酶联免疫吸附法检测白细胞介素(IL)-10,比较两组外周血Tregs比例和IL-10水平.结果 观察组外周血Tregs比例治疗后1周较治疗前明显降低[(5.07±0.82)％比(11.44±2.74)％](P＜0.01),与对照组的(2.45±0.67)％比较差异均有统计学意义(P＜0.01).观察组外周血IL-10水平治疗后1周较治疗前明显降低[(25.47±5.43) mg/L比(41.66±9.24) mg/L](P＜ 0.01),与对照组的(14.32±3.64) mg/L比较差异有统计学意义(P＜0.01).结论 肝癌患者存在Tregs比例和IL-10水平的高表达,减低Tregs比例以及下调IL-10水平可能是热消融治疗提高机体抗肿瘤免疫作用的主要机制.
目的 探討肝癌患者熱消融治療對調節性T淋巴細胞(Tregs)的影響及其提高機體抗腫瘤免疫作用的相關機製.方法 選擇2009年12月至2010年12月行微波消融治療的肝癌患者36例(觀察組),併選擇同期健康體檢者20例作為對照組.採用流式細胞儀檢測Tregs;採用酶聯免疫吸附法檢測白細胞介素(IL)-10,比較兩組外週血Tregs比例和IL-10水平.結果 觀察組外週血Tregs比例治療後1週較治療前明顯降低[(5.07±0.82)％比(11.44±2.74)％](P＜0.01),與對照組的(2.45±0.67)％比較差異均有統計學意義(P＜0.01).觀察組外週血IL-10水平治療後1週較治療前明顯降低[(25.47±5.43) mg/L比(41.66±9.24) mg/L](P＜ 0.01),與對照組的(14.32±3.64) mg/L比較差異有統計學意義(P＜0.01).結論 肝癌患者存在Tregs比例和IL-10水平的高錶達,減低Tregs比例以及下調IL-10水平可能是熱消融治療提高機體抗腫瘤免疫作用的主要機製.
목적 탐토간암환자열소융치료대조절성T림파세포(Tregs)적영향급기제고궤체항종류면역작용적상관궤제.방법 선택2009년12월지2010년12월행미파소융치료적간암환자36례(관찰조),병선택동기건강체검자20례작위대조조.채용류식세포의검측Tregs;채용매련면역흡부법검측백세포개소(IL)-10,비교량조외주혈Tregs비례화IL-10수평.결과 관찰조외주혈Tregs비례치료후1주교치료전명현강저[(5.07±0.82)％비(11.44±2.74)％](P＜0.01),여대조조적(2.45±0.67)％비교차이균유통계학의의(P＜0.01).관찰조외주혈IL-10수평치료후1주교치료전명현강저[(25.47±5.43) mg/L비(41.66±9.24) mg/L](P＜ 0.01),여대조조적(14.32±3.64) mg/L비교차이유통계학의의(P＜0.01).결론 간암환자존재Tregs비례화IL-10수평적고표체,감저Tregs비례이급하조IL-10수평가능시열소융치료제고궤체항종류면역작용적주요궤제.
Objective To investigate the changes of regulatory T cells (Tregs) in hepatocellular carcinoma(HCC) patients with thermal ablation therapy(TAT),and to explore the mechanisms of immunity enhancement induced by TAT.Methods A total of 36 patients with HCC undergoing TAT (observation group) were enrolled in this study from December 2009 to December 2010.Another 20 healthy person were selected as control group.The percent of Tregs was detected by flow cytometry analysis.The level of serum IL-10 was measured with ELISA.Results Before treatment,the percent of Tregs in TAT group was significantly higher than that in control group [(11.44 ± 2.74)％ vs.(2.45 ± 0.67)％] (P ＜ 0.01).After treatment,the percent of Tregs in TAT group decreased significantly[(11.44 ± 2.74)％ vs.(5.07 ± 0.82)％] (P ＜ 0.01).The level of serum IL-10 in TAT group [(41.66 ± 9.24) mg/L] was significantly higher than that in control group [(14.32 ± 3.64) mg/L] (P ＜ 0.01).After treatment,the level of serum IL-10 in TAT group decreased significantly[(41.66 ± 9.24) mg/L vs.(25.47 ± 5.43) mg/L] (P ＜ 0.01).Conclusion TAT can enhance immunity in HCC patients,and which may accomplish by down regulation of Treg and IL-10.
