CAJ | 학술논문

目的：评价扶正化瘀方对不同病理阶段四氯化碳（CCl4）大鼠肝纤维化及活化星状细胞的影响。方法：用CCl4复合法制备大鼠肝纤维化模型，干预组分别于造模之日、2周末、4周末起以扶正化瘀方稀释液灌胃，在第2、4、6周末分批处死大鼠，观察各组大鼠肝组织病理学，采用计算机图像分析测定大鼠肝组织纤维化面积比例；测定α-平滑肌肌动蛋白（α-SMA）的积分光密度（IOD）值。组间数据比较用完全随机设计的单因素方差分析。结果：随着造模时间的延长，与正常组比较，2、4、6周末模型组、各干预组肝组织纤维化面积比例明显升高，α-SMA表达亦均有升高（P＜0．05）；与模型组比较，各干预组大鼠肝组织纤维化面积比例均减少，大鼠肝组织α-SMA的表达均有下调，且均为药物干预一组下降显著（P＜0．05）。结论：扶正化瘀方在不同病理阶段均减少了α-SMA的表达，具有抗肝纤维化的作用，且越早进行干预治疗效果越好。
목적：평개부정화어방대불동병리계단사록화탄（CCl4）대서간섬유화급활화성상세포적영향。방법：용CCl4복합법제비대서간섬유화모형，간예조분별우조모지일、2주말、4주말기이부정화어방희석액관위，재제2、4、6주말분비처사대서，관찰각조대서간조직병이학，채용계산궤도상분석측정대서간조직섬유화면적비례；측정α-평활기기동단백（α-SMA）적적분광밀도（IOD）치。조간수거비교용완전수궤설계적단인소방차분석。결과：수착조모시간적연장，여정상조비교，2、4、6주말모형조、각간예조간조직섬유화면적비례명현승고，α-SMA표체역균유승고（P＜0．05）；여모형조비교，각간예조대서간조직섬유화면적비례균감소，대서간조직α-SMA적표체균유하조，차균위약물간예일조하강현저（P＜0．05）。결론：부정화어방재불동병리계단균감소료α-SMA적표체，구유항간섬유화적작용，차월조진행간예치료효과월호。
Objective To evaluate the influence of Fuzheng Huayu decoction on different pathological stages fibrotic liver tissue and activated hepatic stellate cells (HSCs). Methods Use a carbon tetrachloride (CCl4)-induced liver cirrhosis rat model system. The drug intervention was administered via oral-gastric irrigation in building, the end of week 2 and 4. Rats were batch sacrificed at the end of week 2 , 4, and 6. Liver fibrosis was evaluated by histology, and expression of a-smooth muscle actin (α-SMA) was determined by immunohistochemistry. Between-group comparisons were made by completely random design and ANOVA with Bonferroni correction. Results At the end of weeks 2, 4 and 6, the model group and drug intervention groups had significantly higher area ratio of liver fibrosis and higher expression of α-SMA than the normal group, but the three drug intervention groups had significantly less area ratio of liver fibrosis and less expression of α-SMA than the model group and the drug intervention 1 group showed the most robust decrease (P<0.05). Conclusion The Fuzheng Huayu decoction leads to down-regulated α-SMA expression and reduced degree of liver fibrosis on different pathological stages of fibrotic liver tissue, and the earlier drug intervention, the better the results.