实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
9期
1363-1366
,共4页
衰老%阿托伐他汀%过氧化物酶体增殖物激活型受体β/δ%白细胞介素-1β%大鼠%心肌细胞
衰老%阿託伐他汀%過氧化物酶體增殖物激活型受體β/δ%白細胞介素-1β%大鼠%心肌細胞
쇠로%아탁벌타정%과양화물매체증식물격활형수체β/δ%백세포개소-1β%대서%심기세포
Aging%Atorvastatin%Peroxisome proliferator activated receptorβ/δ%Interleukin-1β%Rat%Myocyte
目的：观察阿托伐他汀下调老年大鼠心肌细胞白细胞介素-1β（interleukin-1β，IL-1β）表达的作用与过氧化物酶体增殖物激活型受体β/δ（peroxisome proliferator activated receptorβ/δ，PPARβ/δ）信号途径的关系。方法：分离培养24月龄大鼠心肌细胞，分为空白对照组、溶剂对照组、阿托伐他汀组、PPARβ/δ拮抗剂GSK0660＋阿托伐他汀组。分别加入细胞培养液、二甲基亚砜（dimethyl sulfoxide， DMSO）、阿托伐他汀、阿托伐他汀＋GSK0660处理。分别用RT-PCR和Western blot方法检测各组细胞IL-1βmRNA和蛋白含量。结果：（1）与空白对照组相比，溶剂对照组细胞的IL-1βmRNA和蛋白水平均无显著差异（P＞0．05）；（2）与空白对照组相比，阿托伐他汀组的IL-1βmRNA蛋白水平均显著降低（P＜0．01）；（3）阿托伐他汀＋GSK0660组的IL-1βmRNA和蛋白水平均显著高于阿托伐他汀组（P＜0．05或P＜0．01），但仍低于空白对照组（P＜0．05）。结论：阿托伐他汀可激活PPARβ/δ信号通路来下调衰老心肌细胞IL-1β的表达。
目的：觀察阿託伐他汀下調老年大鼠心肌細胞白細胞介素-1β（interleukin-1β，IL-1β）錶達的作用與過氧化物酶體增殖物激活型受體β/δ（peroxisome proliferator activated receptorβ/δ，PPARβ/δ）信號途徑的關繫。方法：分離培養24月齡大鼠心肌細胞，分為空白對照組、溶劑對照組、阿託伐他汀組、PPARβ/δ拮抗劑GSK0660＋阿託伐他汀組。分彆加入細胞培養液、二甲基亞砜（dimethyl sulfoxide， DMSO）、阿託伐他汀、阿託伐他汀＋GSK0660處理。分彆用RT-PCR和Western blot方法檢測各組細胞IL-1βmRNA和蛋白含量。結果：（1）與空白對照組相比，溶劑對照組細胞的IL-1βmRNA和蛋白水平均無顯著差異（P＞0．05）；（2）與空白對照組相比，阿託伐他汀組的IL-1βmRNA蛋白水平均顯著降低（P＜0．01）；（3）阿託伐他汀＋GSK0660組的IL-1βmRNA和蛋白水平均顯著高于阿託伐他汀組（P＜0．05或P＜0．01），但仍低于空白對照組（P＜0．05）。結論：阿託伐他汀可激活PPARβ/δ信號通路來下調衰老心肌細胞IL-1β的錶達。
목적：관찰아탁벌타정하조노년대서심기세포백세포개소-1β（interleukin-1β，IL-1β）표체적작용여과양화물매체증식물격활형수체β/δ（peroxisome proliferator activated receptorβ/δ，PPARβ/δ）신호도경적관계。방법：분리배양24월령대서심기세포，분위공백대조조、용제대조조、아탁벌타정조、PPARβ/δ길항제GSK0660＋아탁벌타정조。분별가입세포배양액、이갑기아풍（dimethyl sulfoxide， DMSO）、아탁벌타정、아탁벌타정＋GSK0660처리。분별용RT-PCR화Western blot방법검측각조세포IL-1βmRNA화단백함량。결과：（1）여공백대조조상비，용제대조조세포적IL-1βmRNA화단백수평균무현저차이（P＞0．05）；（2）여공백대조조상비，아탁벌타정조적IL-1βmRNA단백수평균현저강저（P＜0．01）；（3）아탁벌타정＋GSK0660조적IL-1βmRNA화단백수평균현저고우아탁벌타정조（P＜0．05혹P＜0．01），단잉저우공백대조조（P＜0．05）。결론：아탁벌타정가격활PPARβ/δ신호통로래하조쇠로심기세포IL-1β적표체。
Objective To investigate the correlation between atorvastatin inhibiting Interleukin-1β(IL-1β) expression and peroxisome proliferator activated receptor β/δ (PPARβ/δ) signal channel in myocyte of aging rat. Methods Primary culture of myocyte were got from aging rat. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GSK0660 group, whichwere treated respectively by Cell culture medium, dimethyl sulfoxide (DMSO), atorvastatin, atorvastin plus GSK0660. The expression level of IL-1βmRNA and protein was evaluated by RT-PCR and Western Blot respectively. Results (1)NO difference were found between control group and DMSO group in expression level of IL-1βmRNA and protein (P>0.05);(2)The expression level of IL-1βmRNA and protein in atorvastatin group were significantly lower than those of control group (P < 0.01); (3)Both mRNA and protein expression level of IL-1βin atorvastatin plus GSK0660 group were higher than those of atorvastatin group(P<0.05 or P<0.01), but still lower than those of control group(P<0.05). Conclusions Atorvastatin down-regulate the expression of IL-1βin aging myocytes by activating PPARβ/δsignal Channel.
