CAJ | 학술논문

以博落回叶片为外植体材料，在MS培养基的基础上添加不同的激素进行愈伤组织诱导试验，并采用液相色谱-质谱法检测愈伤组织中的血根碱含量，比较不同愈伤组织中的血根碱代谢累积差异。结果表明：Y-9培养基（添加0.75 mg/L 6-BA和0.5 mg/L NAA）上所生长的愈伤组织中血根碱含量最高，达到0.1910 mg/mL；添加NAA的Y-9培养基中的愈伤组织血根碱代谢累积水平较高，为XY-1培养基中愈伤组织血根碱含量的18倍以上，也明显高于其他培养基中愈伤组织血根碱的含量；高浓度的2,4-D不利于血根碱的代谢累积。
이박락회협편위외식체재료，재MS배양기적기출상첨가불동적격소진행유상조직유도시험，병채용액상색보-질보법검측유상조직중적혈근감함량，비교불동유상조직중적혈근감대사루적차이。결과표명：Y-9배양기（첨가0.75 mg/L 6-BA화0.5 mg/L NAA）상소생장적유상조직중혈근감함량최고，체도0.1910 mg/mL；첨가NAA적Y-9배양기중적유상조직혈근감대사루적수평교고，위XY-1배양기중유상조직혈근감함량적18배이상，야명현고우기타배양기중유상조직혈근감적함량；고농도적2,4-D불리우혈근감적대사루적。
Taking the leaf of Macleaya cordata as explant material and adding different hormones to the MS culture medium to conduct callus inducement experiment, and then using UPLC-QTOF to detect the sanguinarine content in callus in order to compare the difference in metabolizing and accumulation of sanguinarine between different calluses. The results showed that the callus grew in Y-9 medium (added with 0.75 mg/L of 6-BA and 0.5 mg/L of NAA) had the highest sanguinarine content, which reached 0.191 0 mg/mL; the metabolizing and accumulation level of sanguinarine in callus grew in Y-9 medium which added with NAA was relatively higher, which was 18-fold of that in callus grew in XY-1 medium, and it also significantly higher than that in callus grew in the other media; high content of 2,4-D was not beneficial for metabolizing and accumulation of sanguinarine.