河北医学
河北醫學
하북의학
HEBEI MEDICINE
2014年
9期
1411-1414,1415
,共5页
禽流感%诊断%比较
禽流感%診斷%比較
금류감%진단%비교
Avian influenza virus%Diagnosis%Comparison
目的:比较4种商品化的甲型/乙型流感病毒抗原检测试剂盒对甲型H6N2、H7N3和H9N2禽流感病毒抗原的检测效果。方法:以H6N2、H7N3、H9N2禽流感毒株作为检测毒株,以倍比稀释法将毒液稀释成不同浓度,严格按照试剂盒说明进行操作,观察这4种试剂盒(3种胶体金法和1种免疫渗滤法)对甲型H6N2、H7N3和H9N2禽流感病毒株的检出情况并比较其对毒株的检测下限。结果:4种试剂盒均可检测出甲型H6N2、H7N3和H9N2禽流感病毒抗原,检测下限范围( log10 TCID50/mL)分别为国产1:1.6~2.4,国产2:3.3~4.1,国产3:3.3~4.1,进口:2.3~2.8。结论:4种试剂盒均可检出甲型H6N2、H7N3和H9N2禽流感病毒抗原;国产1的检测效果较好,操作简便快捷。这些检测试剂都可考虑作为禽流感可疑病例的筛查使用。
目的:比較4種商品化的甲型/乙型流感病毒抗原檢測試劑盒對甲型H6N2、H7N3和H9N2禽流感病毒抗原的檢測效果。方法:以H6N2、H7N3、H9N2禽流感毒株作為檢測毒株,以倍比稀釋法將毒液稀釋成不同濃度,嚴格按照試劑盒說明進行操作,觀察這4種試劑盒(3種膠體金法和1種免疫滲濾法)對甲型H6N2、H7N3和H9N2禽流感病毒株的檢齣情況併比較其對毒株的檢測下限。結果:4種試劑盒均可檢測齣甲型H6N2、H7N3和H9N2禽流感病毒抗原,檢測下限範圍( log10 TCID50/mL)分彆為國產1:1.6~2.4,國產2:3.3~4.1,國產3:3.3~4.1,進口:2.3~2.8。結論:4種試劑盒均可檢齣甲型H6N2、H7N3和H9N2禽流感病毒抗原;國產1的檢測效果較好,操作簡便快捷。這些檢測試劑都可攷慮作為禽流感可疑病例的篩查使用。
목적:비교4충상품화적갑형/을형류감병독항원검측시제합대갑형H6N2、H7N3화H9N2금류감병독항원적검측효과。방법:이H6N2、H7N3、H9N2금류감독주작위검측독주,이배비희석법장독액희석성불동농도,엄격안조시제합설명진행조작,관찰저4충시제합(3충효체금법화1충면역삼려법)대갑형H6N2、H7N3화H9N2금류감병독주적검출정황병비교기대독주적검측하한。결과:4충시제합균가검측출갑형H6N2、H7N3화H9N2금류감병독항원,검측하한범위( log10 TCID50/mL)분별위국산1:1.6~2.4,국산2:3.3~4.1,국산3:3.3~4.1,진구:2.3~2.8。결론:4충시제합균가검출갑형H6N2、H7N3화H9N2금류감병독항원;국산1적검측효과교호,조작간편쾌첩。저사검측시제도가고필작위금류감가의병례적사사사용。
Objective:Comparing the four commercially available influenza virus rapid test kits for de-tection effect of H6N2, H7N3, H9N2 avian influenza viruses .Method:H6N2,H7N3,H9N2 avian influenza viruses solution was diluted into different concentration using 1:1-dilution method .According to the instruc-tions of kits, the different concentrations of H6N2,H7N3,H9N2 avian influenza viruses were detected by four influenza virus rapid test kits (Colloidal Gold Immunoassay and Dot-ELISA).Additionally, the limit of de-tection of the four influenza virus rapid test kits was determined and compared .Result:Four influenza virus rapid test kits can detect H 6N2 H7N3 and H9N2 avian influenza virus antigen .The range of limit of detection (log10TCID50/mL) including home-made 1, home-made 2, home-made 3 and imported were 1.6~2.4, 3. 3~4.1, 3.3~4.1, 2.3~2.8, respectively.Conclusion:Four influenza virus rapid test kits can detect H 6N2 H7N3 and H9N2 avian influenza virus antigen .The detection effect of home-made 1 is better than other kits. Meanwhile the influenza virus rapid test kit is a fast assay .The four influenza virus rapid test kits are consid-ered as the clinical screening of avian influenza virus .