CAJ | 학술논문

以吴茱萸挥发油为研究对象，进行空气抑菌、平板抑菌、液体培养等试验，并通过气相色谱-质谱（GC-MS）联用技术对吴茱萸挥发油进行化学成分分析。结果表明：吴茱萸挥发油对空气中的细菌和真菌的抑菌率分别为97.86%和99.50%；对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌均有抑制效果，但对革兰氏阴性菌的抑制效果较弱，而对革兰氏阳性菌的抑制效果较强；经4.5%的挥发油作用24 h后，金黄色葡萄球菌细胞与细胞间界限模糊，菌体结成团块，并且有不规则凸起或凹陷，结构破坏严重，菌内物质外漏，最后裂解成碎片。GC-MS检测的色谱峰超过100个，经鉴定吴茱萸挥发油主要成分为月桂烯、β-蒎烯、柠檬烯及罗勒烯，占总成分的78.66%。
이오수유휘발유위연구대상，진행공기억균、평판억균、액체배양등시험，병통과기상색보-질보（GC-MS）련용기술대오수유휘발유진행화학성분분석。결과표명：오수유휘발유대공기중적세균화진균적억균솔분별위97.86%화99.50%；대대장간균、금황색포도구균화고초아포간균균유억제효과，단대혁란씨음성균적억제효과교약，이대혁란씨양성균적억제효과교강；경4.5%적휘발유작용24 h후，금황색포도구균세포여세포간계한모호，균체결성단괴，병차유불규칙철기혹요함，결구파배엄중，균내물질외루，최후렬해성쇄편。GC-MS검측적색보봉초과100개，경감정오수유휘발유주요성분위월계희、β-파희、저몽희급라륵희，점총성분적78.66%。
The experiments of air bacteriostasis, plate bacteriostasis and liquid culture were conducted to the volatile oil of Evodia rutaecarpa, and then the chemical components of the volatile oil of Evodia rutaecarpa were analyzed by GC-MS. The results showed that the bacteriostatic rate of the volatile oil of Evodia rutaecarpa to bacteria and fungi in the air reached 97.86% and 99.50%, respectively;while it also showed a bacteriostatic effect to Escherichia coli, Staphylococcus aureus and Bacillus subtilis, a strong bacteriostatic effect to Gram-negative bacteria, but a weak bacteriostatic effect to Gram-negative bacteria; treated with 4.5% volatile oil for 24 h, the boundary between cells of Staphylococcus aureus was indistinct, the thalli formed a block mass with irregular heave or depression, and the structure damaged badly and substance in bacterial leaked out, and then split into pieces. Over 100 chromatographic peaks were detected by GC-MS, and the main chemical components of Evodia rutaecarpa volatile oil were identified as myrcene, β-pinene, limonene and ocimene, which accounts for 78.66% of total components.