湖南农业科学
湖南農業科學
호남농업과학
HUNAN AGRICULTURAL SCIENCES
2014年
10期
13-15,18
,共4页
王英姿%蒋端生%蒋祖丰%段祥石
王英姿%蔣耑生%蔣祖豐%段祥石
왕영자%장단생%장조봉%단상석
铁皮石斛%组织培养%果蔬添加物%茎尖组织
鐵皮石斛%組織培養%果蔬添加物%莖尖組織
철피석곡%조직배양%과소첨가물%경첨조직
Dendrobium officinale%tissue culture%fruit and vegetable additives%stem-tip tissue
在铁皮石斛茎尖组织培养基中分别添加10%、20%和30%的椰汁、香蕉、苹果、红薯、番茄和马铃薯6种果蔬添加物,研究其对铁皮石斛茎尖组织生长情况的影响。结果表明:除香蕉外,其他果蔬添加物对铁皮石斛丛生芽的生长均表现出一定的促进作用,其添加最适浓度有明显差异。其中,苹果和红薯均以30%的添加浓度最为适宜,番茄和椰子则分别以20%和10%的浓度较为适宜,而马铃薯添加10%和20%的差异不明显,对铁皮石斛茎尖组织的生长促进效果较好。
在鐵皮石斛莖尖組織培養基中分彆添加10%、20%和30%的椰汁、香蕉、蘋果、紅藷、番茄和馬鈴藷6種果蔬添加物,研究其對鐵皮石斛莖尖組織生長情況的影響。結果錶明:除香蕉外,其他果蔬添加物對鐵皮石斛叢生芽的生長均錶現齣一定的促進作用,其添加最適濃度有明顯差異。其中,蘋果和紅藷均以30%的添加濃度最為適宜,番茄和椰子則分彆以20%和10%的濃度較為適宜,而馬鈴藷添加10%和20%的差異不明顯,對鐵皮石斛莖尖組織的生長促進效果較好。
재철피석곡경첨조직배양기중분별첨가10%、20%화30%적야즙、향초、평과、홍서、번가화마령서6충과소첨가물,연구기대철피석곡경첨조직생장정황적영향。결과표명:제향초외,기타과소첨가물대철피석곡총생아적생장균표현출일정적촉진작용,기첨가최괄농도유명현차이。기중,평과화홍서균이30%적첨가농도최위괄의,번가화야자칙분별이20%화10%적농도교위괄의,이마령서첨가10%화20%적차이불명현,대철피석곡경첨조직적생장촉진효과교호。
Adding six fruit and vegetable additives (coconut juice, banana, apple, sweet-potato, tomato and potato) with 10%, 20% and 30% to Dendrobium officinale stem-tip tissue culture media, respectively, in order to study the effects of different fruit and vegetable additives on growth of Dendrobium officinale stem-tip tissue. The results showed that except banana, the other fruit and vegetable additives all showed a certain promoting effect on growth of axillary buds, but their optimal adding content had significant difference, among which the optimal adding content for apple and sweet-potato was 30%, and for tomato and coconut juice was 20% and 10%, respectively, moreover, there was no significant difference in 10% and 20% adding content for potato.