CAJ | 학술논문

目的：观察甲状腺素对体外培养的人脐静脉血管内皮细胞（ HUVECs ）增殖、迁移的影响。方法采用噻唑兰比色法检测各浓度（0、10-10、10-9、10-8、10-7、10-6 mmol/L）甲状腺素对HUVECs增殖的影响；细胞划痕实验及Transwell实验检测甲状腺素（0、10-9、10-7 mmol/L）对HUVECs迁移的影响；利用原子力显微镜（ AFM）观察甲状腺素（0、10-9、10-7 mmol/L）对HUVECs 细胞表面超微结构的影响。结果与0 mmol/L相比，10-10、10-9、10-8、10-7、10-6 mmol/L甲状腺素处理的HUVECs的增殖率明显增大，增殖率分别为（11.572±0.68）％、（18.549±7.53）％、（24.183±4.02）％、（38.724±1.98）％、（32.492±0.76）％，并且细胞增殖程度与甲状腺素浓度（10-10～10-7 mmol/L）呈剂量依赖关系（P＜0.01）；10-9、10-7 mmol/L甲状腺素处理的HUVECs的迁移距离和迁移数量均较0 mmol/L明显增大（ P＜0.01），迁移距离和迁移数量依处理浓度的由低到高分别为（18.649±2.370）、（58.176±4.163）、（140.354±25.239）μm和（37.673±2.302）、（73.860±2.608）、（117.405±5.128）个；甲状腺素可使HUVECs 表面超微结构发生明显改变，表现为细胞表面粗糙度增加，突起增大及数量增多。结论甲状腺素可以改变HUVECs表面的超微结构，促进血管内皮细胞形成突起及伪足，进一步促进血管内皮细胞的增殖、迁移。
목적：관찰갑상선소대체외배양적인제정맥혈관내피세포（ HUVECs ）증식、천이적영향。방법채용새서란비색법검측각농도（0、10-10、10-9、10-8、10-7、10-6 mmol/L）갑상선소대HUVECs증식적영향；세포화흔실험급Transwell실험검측갑상선소（0、10-9、10-7 mmol/L）대HUVECs천이적영향；이용원자력현미경（ AFM）관찰갑상선소（0、10-9、10-7 mmol/L）대HUVECs 세포표면초미결구적영향。결과여0 mmol/L상비，10-10、10-9、10-8、10-7、10-6 mmol/L갑상선소처리적HUVECs적증식솔명현증대，증식솔분별위（11.572±0.68）％、（18.549±7.53）％、（24.183±4.02）％、（38.724±1.98）％、（32.492±0.76）％，병차세포증식정도여갑상선소농도（10-10～10-7 mmol/L）정제량의뢰관계（P＜0.01）；10-9、10-7 mmol/L갑상선소처리적HUVECs적천이거리화천이수량균교0 mmol/L명현증대（ P＜0.01），천이거리화천이수량의처리농도적유저도고분별위（18.649±2.370）、（58.176±4.163）、（140.354±25.239）μm화（37.673±2.302）、（73.860±2.608）、（117.405±5.128）개；갑상선소가사HUVECs 표면초미결구발생명현개변，표현위세포표면조조도증가，돌기증대급수량증다。결론갑상선소가이개변HUVECs표면적초미결구，촉진혈관내피세포형성돌기급위족，진일보촉진혈관내피세포적증식、천이。
Objective To observe the effects of thyroxine hormone ( TH) on the proliferation and migration of human umbilical vein endothelial cells (HUVECs).Methods HUVECs were co-cultured with TH.The proliferation rate of HUVECs was examined by MTT assay , while the cell migration ability was assessed by using the modified of wound healing assay and transwell chamber assay .The morphology and ultrastructure observation of HUVECs membrane surface was carried out by atomic force microscope ( AFM) .Results TH significantly induced proliferation of HUVECs in a dose -dependent manner ( P<0.01 ) .The proliferation rates of HUVECs in each group ( 10 -10 , 10 -9 , 10 -8 10-7 and 10 -6 mmol/L) were (11.572 ±0.68)%, (18.549 ±7.53)%, (24.183 ±4.02)%, (38.724 ±1.98)% and (32.492 ± 0.76)%, respectively.The migration distance of HUVECs and the number of cells passing the transwell chamber were both significantly increased in the groups treated with TH than in control group (0 mmol/L) (P<0.01).The migration distances in control, 10-9 mmol/L and 10-7 mmol/L groups were (18.649 ±2.370), (58.176 ±4.163) and (140.354 ± 25.239)μm, respectively;while the numbers of HUVECs passing the transwell chamber were 37.673 ±2.302, 73.860 ± 2.608 and 117.405 ±5.128, respectively.According to AFM image, HUVECs were more active after the treatment with TH than the control group (0 mmol/L) cells, presenting with more pseudopodium , granules extended the edges of cell and rough surface in cell membrane .Conclusion TH can change the ultrastructure of the HUVECs surface thus to promote cell proliferation and migration .